Improvement The Quality of 'Soya Bean Seed Storage Proteins by Genetic Engineering Cdna Encoding G2 Subunit

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Author Sammour, Reda Helmy [رضا حلمي سمور] en_US
Available date 2009-11-25T15:29:47Z en_US
Publication Date 2003 en_US
Citation Qatar University Science Journal, 2003, Vol. 23, Pages 87-98. en_US
URI http://hdl.handle.net/10576/10199 en_US
Abstract The goal of this work is to improve nutritional quality of soybean seed proteins by altering cDNA-encoding G2 subunit of Soybean glycinin to be capable of self-assembly in vitro. Two plasmids were constructed; one designated pSP65/G4SacG2 and the other pSP65/G45<7cG2HG4. The observation that basic chain did not tolerate modification and that a 21 amino acids deletion in the basic chain of G4 proglycinin inhibited self-assembly into trimers [I], make the introduction of Sac fragment of GY4 to GY2 is a possible key factor in getting G2 self-assemble. pSP65/G45acG2HG4 construct includes, in addition to G4 Sac fragment, the acidic chain of GY4 which was proved to tolerate an introduction of three Met residues. This construct, therefore, had the ability to self-assemble in vitro and in the mean time the ability to harbor a number of Met residues. The ability of these constructs to assemble into oligomer similar to those that occur in the seed was tested. en_US
Abstract يهدف هذا البحث إلى تحسين نوعية بروتينات بذور فول الصويا، من خاجل تغير اد"دنا" (المكمل ) المشنر لتحت وحدة الجليسنين "جى 2"، مما يجعلها قادرة على التجمع في المعمل . لإحداث ذلك ، استخدمت تقنيات البيولوجيا الجزيئية التالية : النزجمة والنسخ في المعمل ، النسخ في المعمل ، النسخ والتجميع الذاقي في . ar
Language en en_US
Publisher Qatar University en_US
Subject Biological Science en_US
Subject بيولوجيا ar
Title Improvement The Quality of 'Soya Bean Seed Storage Proteins by Genetic Engineering Cdna Encoding G2 Subunit en_US
Alternative Title تحسين نوعية البروتينات المخزنة ببذور فول الصويا من خلال هندسة الـ"دنا" (المكمل ) المشفر لتحت وحدة الجليسنين "جى 2" ar
Type Article en_US
Pagination 87-98 en_US
Volume Number 23 en_US


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