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AuthorAbdulrahman, Nabeel
AuthorJaballah, Maiy
AuthorPoomakkoth, Noufira
AuthorRiaz, Sadaf
AuthorAbdelaziz, Somaia
AuthorIssa, Aya
AuthorMraiche, Fatima
Available date2017-01-18T10:45:11Z
Publication Date2016-07
Publication NameMolecular and Cellular Biochemistryen_US
Identifierhttp://dx.doi.org/10.1007/s11010-016-2727-9
CitationNabeel Abdulrahman, Maiy Jaballah, Noufira Poomakkoth, Sadaf Riaz, Somaia Abdelaziz, Aya Issa, Fatima Mraiche "Inhibition of p90 ribosomal S6 kinase attenuates cell migration and proliferation of the human lung adenocarcinoma through phospho-GSK-3β and osteopontin" (2016) Molecular and Cellular Biochemistry vol 418 issue 1 pages 21-29
ISSN0300-8177
URIhttp://hdl.handle.net/10576/5193
Abstractp90 ribosomal S6 kinase (p90RSK) constitutes a family of serine/threonine kinases that have been shown to be involved in cell proliferation of various malignancies via direct or indirect effects on the cell-cycle machinery. We investigated the role of p90RSK in lung adenocarcinomas and whether the inhibition of p90RSK diminishes cancer progression. Moreover, we investigated the involvement of glycogen synthase kinase-3β (GSK-3β) and osteopontin (OPN) in the p90RSK-induced lung adenocarcinoma progression. p90RSK, OPN, and GSK-3β protein expressions were examined in the A549 human lung adenocarcinoma cell line in the presence and absence of BI-D1870 (BID), a p90RSK inhibitor. Gene expression of anti-apoptotic and pro-apoptotic markers namely Bcl2 and Bax, respectively, were studied by reverse transcription polymerase chain reaction. In addition, the A549 lung adenocarcinoma cell line was characterized for cell proliferation using the MTT assay and cell migration using the scratch migration assay. Our study revealed that total RSK1 protein expression is over expressed in the A549 human lung adenocarcinoma cell line, an effect which is significantly reduced upon pretreatment with BID (69.32 ± 12.41 % of control; P < 0.05). The inhibition of p90RSK also showed a significant suppression of cell proliferation (54.3 ± 6.73 % of control; P < 0.01) and cell migration (187.90 ± 16.10 % of control; P < 0.01). Treatment of the A549 cells with BID regressed the expression of Bcl2 mRNA (56.92 ± 6.07 % of control; P < 0.01). BID also regressed protein expression of OPN (79.57 ± 5.32 % of control; P < 0.05) and phospho-GSK-3β (73.04 ± 8.95 % of control; P < 0.05). The p90RSK has an essential role in promoting tumor growth and proliferation in non-small cell lung cancer (NSCLC). BID may serve as an alternative cancer treatment in NSCLC.
SponsorThis work was supported by a Qatar University Internal Grant (QU-UG2013/2014).
Languageen
PublisherSpringer-Verlag
Subjectp90 Ribosomal S6 kinase
SubjectLung adenocarcinoma
Subjectglycogen synthase kinase-3β
SubjectOsteopontin
SubjectCell proliferation
SubjectCell migration
SubjectAntiapoptosis
TitleInhibition of p90 ribosomal S6 kinase attenuates cell migration and proliferation of the human lung adenocarcinoma through phospho-GSK-3β and osteopontin
TypeArticle
Pagination21-29
Issue Number1
Volume Number418
dc.identifier.essn 1573-4919


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