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Author Abdulrahman, Nabeel
Author Jaballah, Maiy
Author Poomakkoth, Noufira
Author Riaz, Sadaf
Author Abdelaziz, Somaia
Author Issa, Aya
Author Mraiche, Fatima
Available date 2017-01-18T10:45:11Z
Publication Date 2016-07
Publication Name Molecular and Cellular Biochemistryen_US
Citation Nabeel Abdulrahman, Maiy Jaballah, Noufira Poomakkoth, Sadaf Riaz, Somaia Abdelaziz, Aya Issa, Fatima Mraiche "Inhibition of p90 ribosomal S6 kinase attenuates cell migration and proliferation of the human lung adenocarcinoma through phospho-GSK-3β and osteopontin" (2016) Molecular and Cellular Biochemistry vol 418 issue 1 pages 21-29en_US
ISSN 0300-8177
Abstract p90 ribosomal S6 kinase (p90RSK) constitutes a family of serine/threonine kinases that have been shown to be involved in cell proliferation of various malignancies via direct or indirect effects on the cell-cycle machinery. We investigated the role of p90RSK in lung adenocarcinomas and whether the inhibition of p90RSK diminishes cancer progression. Moreover, we investigated the involvement of glycogen synthase kinase-3β (GSK-3β) and osteopontin (OPN) in the p90RSK-induced lung adenocarcinoma progression. p90RSK, OPN, and GSK-3β protein expressions were examined in the A549 human lung adenocarcinoma cell line in the presence and absence of BI-D1870 (BID), a p90RSK inhibitor. Gene expression of anti-apoptotic and pro-apoptotic markers namely Bcl2 and Bax, respectively, were studied by reverse transcription polymerase chain reaction. In addition, the A549 lung adenocarcinoma cell line was characterized for cell proliferation using the MTT assay and cell migration using the scratch migration assay. Our study revealed that total RSK1 protein expression is over expressed in the A549 human lung adenocarcinoma cell line, an effect which is significantly reduced upon pretreatment with BID (69.32 ± 12.41 % of control; P < 0.05). The inhibition of p90RSK also showed a significant suppression of cell proliferation (54.3 ± 6.73 % of control; P < 0.01) and cell migration (187.90 ± 16.10 % of control; P < 0.01). Treatment of the A549 cells with BID regressed the expression of Bcl2 mRNA (56.92 ± 6.07 % of control; P < 0.01). BID also regressed protein expression of OPN (79.57 ± 5.32 % of control; P < 0.05) and phospho-GSK-3β (73.04 ± 8.95 % of control; P < 0.05). The p90RSK has an essential role in promoting tumor growth and proliferation in non-small cell lung cancer (NSCLC). BID may serve as an alternative cancer treatment in NSCLC.en_US
Sponsor This work was supported by a Qatar University Internal Grant (QU-UG2013/2014).en_US
Language enen_US
Publisher Springer-Verlagen_US
Subject p90 Ribosomal S6 kinaseen_US
Subject Lung adenocarcinomaen_US
Subject glycogen synthase kinase-3βen_US
Subject Osteopontinen_US
Subject Cell proliferationen_US
Subject Cell migrationen_US
Subject Antiapoptosisen_US
Title Inhibition of p90 ribosomal S6 kinase attenuates cell migration and proliferation of the human lung adenocarcinoma through phospho-GSK-3β and osteopontinen_US
Type Articleen_US
Pagination 21-29
Issue Number 1
Volume Number 418

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