A single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay
| Author | Mansour, Amira |
| Author | Tammam, Salma |
| Author | Althani, Asma |
| Author | Azzazy, Hassan M.E. |
| Available date | 2017-07-30T07:32:46Z |
| Publication Date | 2017-08-01 |
| Publication Name | Journal of Microbiological Methods |
| Identifier | http://dx.doi.org/10.1016/j.mimet.2017.06.001 |
| Citation | Mansour A, Tammam S, Althani A, Azzazy HME. A single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay. Journal of Micobiological Methods. Vol. 139, P.p. 139. |
| ISSN | 01677012 |
| Abstract | Abstract The global combat against MTB is limited by challenges in accurate affordable detection. In this study, a rapid, affordable, single tube system for detection of unamplified MTB16s rDNA was developed. Utilizing a AuNP based FRET system, this assay achieved a sensitivity and specificity of 98.6% and 90% respectively. |
| Sponsor | This report was made possible by a NPRP award [NPRP 4-1215-3- 317] from the Qatar National Research Fund (a member of The Qatar Foundation). |
| Language | en |
| Publisher | Elsevier |
| Subject | Mycobacterium tuberculosis Fluorescence Resonance Energy Transfer Gold nanoparticles 16s rDNA In vitro diagnosis |
| Type | Article |
| Pagination | 165-167 |
| Volume Number | 139 |
| ESSN | 1872-8359 |
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