Regeneration of Transformed Forage Legume Plants by Agrobacterium rhizogenes R1601

Abstract

Transformed forage legume plants (alfalfa & clover) by genetic engineering bacteria Agrobacterium rhizogenes R1601,were regenerated directly from seedling plants after inoculating the wounded site formed by cutting the plantlet stem at the cotyledon attachment site , and from hairy roots which formed on the hypocotyledon stem of these seedlings . Results show transgenic plants have large number of transformed shoots , with curled leaves compared to the control samples (seedlings plants),which were assayed by investigating the presence of the agropine using high voltage paper electrophoresis.