Use of β-cyclodextrin as enhancer of ascorbic acid rejection in permselective films for amperometric biosensor applications
Author | Monti P. |
Author | Rocchitta G. |
Author | Marceddu S. |
Author | Dettori M.A. |
Author | Fabbri D. |
Author | Jaoua S. |
Author | Migheli Q. |
Author | Delogu G. |
Author | Serra P.A. |
Available date | 2020-03-18T08:10:07Z |
Publication Date | 2018 |
Publication Name | Talanta |
Resource | Scopus |
ISSN | 399140 |
Abstract | Interference rejection in amperometric biosensors can be more effective introducing some modifiers during electro-deposition of permselective film. Addition of β-cyclodextrin (βCD), a cyclic oligosaccharide composed of seven glucose units, to the ortho-phenylendiamine (oPD) monomer were already demonstrated to provide an enhancement in ascorbic acid (AA) rejection. Here we evaluated the improvement in permselectivity of poly-eugenol and poly-magnolol films electro-polymerized in presence of different amounts of βCD or eugenol-βCD inclusion complex for amperometric biosensor application. Starting from Pt-Ir wire as transducer several microsensors were covered with polymeric films doped with βCD-based modifiers through constant potential amperometry. Characterization of modified polymers was achieved by scanning electron microscopy and permselectivity analysis. Poly-magnolol film in combination with βCD showed a worsening in permselectivity compared to poly-magnolol alone. In contrast, the introduction of βCD-based modifier enhanced the interference rejection toward the archetypal interferent AA, while slightly affecting permeability toward H2O2 compared to the poly-eugenol without modifier. The AA rejection seems to be influenced by the availability of βCD cavity as well as film performance due to concentration of βCD-Eugenol inclusion complex. A poly-eugenol film co-polymerized with 2 mM βCD-eugenol inclusion complex showed a permselectivity equal to poly-orthophenylendiamine film (PPD), with a lower permeability to AA, likely to be related with a self-blocking mechanism. Based on these results, a biosensor for glutamate was constructed with a poly-eugenol doped with βCD-eugenol as permselective layer and its permselectivity, stability and lifetime were determined. © 2018 |
Sponsor | This work was supported by the Qatar National Research Fund (a member of Qatar Foundation) [NPRP grant # 8-392-4-003 ]. |
Language | en |
Publisher | Elsevier B.V. |
Subject | Co-polymerization Electro-polymerization Eugenol Inclusion complex Self-blocking β-cyclodextrin |
Type | Article |
Pagination | 53 - 59 |
Volume Number | 186 |
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Biological & Environmental Sciences [919 items ]