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AuthorNayla Salah, Higazy
AuthorSaleh, Aya Ehab
AuthorHassan, Zahoor Ul
AuthorAl Thani, Roda
AuthorMigheli, Quirico
AuthorJaoua, Samir
Available date2022-09-18T07:54:13Z
Publication Date2021-01-31
Publication NameFood Control
Identifierhttp://dx.doi.org/10.1016/j.foodcont.2020.107464
CitationHigazy, N. S., Saleh, A. E., Hassan, Z. U., Al Thani, R., Migheli, Q., & Jaoua, S. (2021). Investigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contamination. Food Control, 119, 107464.‏
ISSN09567135
URIhttps://www.sciencedirect.com/science/article/pii/S0956713520303807
URIhttp://hdl.handle.net/10576/34100
AbstractThis study was designed to investigate the antifungal activity of Bacillus pumilus 344-3 against toxigenic fungi. In vitro co-incubation assay revealed that A. carbonarius AC82, A. niger AN8 and P. digitatum PD43 are most sensitive fungi to bacterial antifungal compounds with zone of inhibition of 29.2 mm, 27.7 mm and 27.1 mm, respectively. The addition of Bacillus pumilus 344-3 culture supernatant at low concentration in the fungal growth medium stimulated A. carbonarius biomass, but inhibited ochratoxin A (OTA) synthesis significantly (p ≤ 0.05). Conidial germination of A. carbonarius was not affected in a medium containing 10% and 20% of the bacterial culture supernatant, while it was completely inhibited in 100% bacterial extract. Storage of bacterial culture supernatant at temperature ranging from −20 °C to 100 °C for 1 h, didn't affect its antifungal potential. In vivo application of bacterial extract on the maize kernels, showed 95% protection against A. carbonarius infection. Application of B. pumilus 344-3 culture supernatant on the surface of maize kernels provided 99% reduction in OTA production potential of A. carbonarius AC82. Because of its strong activities against the growth of A. carbonarius AC82 and OTA-synthesis, B. pumilus 344-3 can be considered as a very promising biocontrol agent.
Languageen
PublisherElsevier Ltd
SubjectBacillus pumilus
Antifungal activity
Mycotoxins
A. carbonarius
Maize
TitleInvestigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contamination
TypeArticle
Volume Number119
Open Access user License http://creativecommons.org/licenses/by/4.0/
dc.accessType Open Access


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