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AuthorRousou, Xanthoula
AuthorFuruya-Kanamori, Luis
AuthorKostoulas, Polychronis
AuthorDoi, Suhail A.R.
Available date2023-02-21T08:44:57Z
Publication Date2022-01-01
Publication NamePathogens and Global Health
Identifierhttp://dx.doi.org/10.1080/20477724.2022.2097830
CitationXanthoula Rousou, Luis Furuya-Kanamori, Polychronis Kostoulas & Suhail A.R. Doi (2022) Diagnostic accuracy of multiplex nucleic acid amplification tests for Campylobacter infection: a systematic review and meta-analysis, Pathogens and Global Health, DOI: 10.1080/20477724.2022.2097830
ISSN20477724
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85133820863&origin=inward
URIhttp://hdl.handle.net/10576/40234
AbstractCampylobacter infection is one of the most frequently reported foodborne diseases with approximately 230,000 and 1.5 million cases each year in Europe and the USA, respectively. Culture methods are the reference for the diagnosis of Campylobacter infections; however, these methods are complex and time-consuming. Multiplex nucleic acid amplification test is favored due to its rapidity, automatization in the procedure followed and the quick simultaneous testing of numerous foodborne pathogens. The aim of this meta-analysis was to evaluate the accuracy of these tests for the diagnosis of Campylobacter infection. Scopus, Science Direct, PubMed, Web of Science, and Mendeley were searched for peer-reviewed articles. The split component synthesis method with the use of the inverse variance heterogeneity model was chosen for the quantitative meta-analysis. Sensitivity analysis was performed by age category and index test. The literature search found 34 studies involving 28,105 patients with suspected gastroenteritis. The sensitivity and specificity were 95.3% (92.3; 97.1) and 97.1% (95.1; 98.3), respectively, and AUC (area under the curve) was 0.963 (0.947; 0.974). Pediatric patients had a lower sensitivity (87.4, 48.2; 98.1) and higher specificity (99.2, 91.6; 99.9) estimate compared to all ages category (sensitivity 95.3, 91.3; 97.5, specificity 96.7, 93.7; 98.3). Among the various index tests, Seeplex/Allplex and Amplidiag/Novodiag had the lowest estimate for sensitivity (88.9, 73.8; 95.8) and specificity (95.2, 86; 98.4), respectively. BDMax had the highest (sensitivity 98.1, 96.1; 99 and specificity 98.5, 97; 99.3). Multiplex nucleic acid tests showed excellent accuracy and could play an influential role in diagnosing Campylobacter infections.
Languageen
PublisherTaylor and Francis Group
Subjectaccuracy
Campylobacter spp
diagnostic odds ratios
multiplex nucleic acid amplification test
PCR
sensitivity
specificity
TitleDiagnostic accuracy of multiplex nucleic acid amplification tests for Campylobacter infection: a systematic review and meta-analysis
TypeArticle Review
ESSN2047-7732


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