A Validated Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for the Quantification of Gamma-Tocotrienol in Tocotrienol Rich Fractions of Crude Palm Oil
Author | Nair, Rajesh Sreedharan |
Author | Billa, Nashiru |
Author | Morris, Andrew |
Available date | 2023-03-29T10:29:40Z |
Publication Date | 2021-08-03 |
Publication Name | Current Nutrition and Food Science |
Identifier | http://dx.doi.org/10.2174/1573401317666210803155717 |
Citation | Nair, R. S., Billa, N., & Morris, A. (2022). A validated reverse-phase high performance liquid chromatography (RP-HPLC) method for the quantification of gamma-tocotrienol in tocotrienol rich fractions of crude palm oil. Current Nutrition & Food Science, 18(1), 75-81. |
ISSN | 1573-4013 |
Abstract | Background: Palm oil and its constituents have wide applications in food, cosmet-ics and pharmaceutical industries. The tocotrienol-rich fractions of crude palm oil have drawn greater research interest in recent years due to their potent health benefits. Therefore, reliable and validated analytical methods are essential for the quantification of tocotrienols. Objective: This study aimed to develop a simple and economical RP-HPLC method for the quantification of gamma-tocotrienol in tocotrienol-rich fractions of crude palm oil. Methods: An Agilent HPLC system supplied with a Diode Array detector and an auto-injector system was used for the method development, and the wavelength was set 295 nm. A reversed-phase C18 column maintained at 30 °C using a mobile phase composition of methanol: water (95:05) at a flow rate of 1 mL/min was used for the analysis. The developed method was validated according to ICH guidelines. Results and Discussion: A symmetrical peak of gamma-tocotrienol was observed at 8.7 minutes with minimal peak tailing (between 0.76 and 0.78), and an acceptable resolution above 2.0. Excellent linearity was evident with R2 values 0.9996 and 0.9991 for intra-day and inter-day, respectively. The method demonstrated a high precision (%RSD values ≤ 5.8%) and accu-racy (%RE<9.6%). The LOD and LOQ of gamma-tocotrienol were determined as 1.4 μg/mL and 4.2 μg/mL, respectively. The system suitability studies indicate that the chromatographic parameters are well within the acceptable limit. Conclusion: In conclusion, the developed RP-HPLC method is rapid, precise, stable and economical for the quantification of gamma-tocotrienol. |
Sponsor | The paper has recieved funding from the Faculty of Sci-ence, University of Nottingham Malaysia, for the financial support provided to this research project. |
Language | en |
Publisher | Bentham |
Subject | Gamma-tocotrienol ICH RP-HPLC System suitability TRF Validation |
Type | Article |
Pagination | 75-81 |
Issue Number | 1 |
Volume Number | 18 |
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