Browsing by Author "Ben Khedher, Saoussen"
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Application of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium
Ben Khedher, Saoussen; Jaoua, Samir; Zouari, Nabil ( Sociedade Brasileira de Microbiologia - SBM , 2014 , Article)In order to overproduce bioinsecticides production by a sporeless Bacillus thuringiensis strain, an optimal composition of a cheap medium was defined using a response surface methodology. In a first step, a Plackett-Burman ... -
Correlation between delta-endotoxin and proteolytic activities produced by Bacillus thuringiensis var. kurstaki growing in an economic production medium
Ennouri, Karim; Ben Khedher, Saoussen; Jaoua, Samir; Zouari, Nabil ( Taylor & Francis , 2013 , Article)Bacillus thuringiensis is a Gram positive bacterium that produces an insecticidal crystalline protein making it one of the most important biocontrol agents for pest management. Bioinsecticides based on B. thuringiensis ... -
Improvement of Bacillus thuringiensis bioinsecticide production by sporeless and sporulating strains using response surface methodology
Ben Khedher, Saoussen; Kamoun, Amel; Jaoua, Samir; Zouari, Nabil ( Elsevier B.V. , 2011 , Article)Statistical experimental designs, involving a Plackett-Burman design followed by a rotatable central composite design were used to optimize the culture medium constituents for Bacillus thuringiensis bioinsecticide production. ... -
Improvement of Bioinsecticides Production by Sporeless Bacillus thuringiensis strains in Response to Various Stresses in Low Cost Medium
Ben Khedher, Saoussen; Jaoua, Samir; Zouari, Nabil ( Springer-Verlag , 2011 , Article)The use of bioinsecticides, particularly those produced by sporeless Bacillus thuringiensis strains, has been shown to be a good alternative in pest management. Two types of sporeless mutants were distinguished. The ... -
Overproduction of Delta-Endotoxins by Sporeless Bacillusthuringiensis Mutants Obtained by Nitrous Acid Mutagenesis
Ben Khedher, Saoussen; Zouari, Nabil; Messaddeq, Nadia; Schultz, Patrick; Jaoua, Samir ( Springer-Verlag , 2011 , Article)Asporogenic and oligosporogenic Bacillus thuringiensis mutants having the ability to overproduce insecticidal crystal protein were generated by using nitrous acid (50 mg/ml), as chemical mutagenic agent. Insecticidal crystal ... -
Overproduction of the Bacillus thuringiensis Vip3Aa16 toxin and study of its insecticidal activity against the carob moth Ectomyelois ceratoniae
Hanen, Boukedi; Ben Khedher, Saoussen; Triki, Nesrine; Kamoun, Fakher; Saadaoui, Imen; Chakroun, Maissa; Tounsi, Slim; Abdelkefi-Mesrati, Lobna... more authors ... less authors ( Elsevier , 2015 , Article)The vip3Aa16 gene of Bacillus thuringiensis strain BUPM95 was cloned and expressed in Escherichia coli. Optimization of Vip3A16 protein expression was conducted using Plackett–Burman design and response surface methodology. ...