Browsing by Author "Nomikos, Michail"
Now showing items 1-20 of 20
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Advancing male age differentially alters levels and localization patterns of PLCzeta in sperm and testes from different mouse strains
( Medknow Publications , 2021 , Article)Sperm-specific phospholipase C zeta (PLC) initiates intracellular calcium (Ca2+) transients which drive a series of concurrent events collectively termed oocyte activation. Numerous investigations have linked abrogation ... -
Antigen Unmasking Is Required to Clinically Assess Levels and Localisation Patterns of Phospholipase C Zeta in Human Sperm
( MDPI , 2023 , Article)Mammalian oocyte activation is initiated by intracellular calcium (Ca2+) oscillations, driven by the testis-specific phospholipase C zeta (PLCζ). Sperm PLCζ analysis represents a diagnostic measure of sperm fertilisation ... -
Arrhythmogenic calmodulin E105A mutation alters cardiac RyR2 regulation leading to cardiac dysfunction in zebrafish
( Wiley , 2019 , Article)Calmodulin (CaM) is a universal calcium (Ca )-binding messenger that regulates many vital cellular events. In cardiac muscle, CaM associates with ryanodine receptor 2 (RyR2) and regulates excitation-contraction coupling. ... -
The Arrhythmogenic E105A CAM Mutation Dysregulates Normal Cardiac Function in Zebrafish by Altering CAM-Ca2+ and CAM-RyR2 Interactions
( Elsevier , 2019 , Article)Calmodulin (CaM) is a multifunctional calcium (Ca2+)-binding messenger that directly interacts with the cardiac ryanodine receptor 2 (RyR2), a large transmembrane Ca2+ channel that mediates Ca2+ release from the sarcoplasmic ... -
Calmodulin Interacts and Regulates Enzyme Activity of the Mammalian Sperm Phospholipase C
( Elsevier , 2017 , Article)Sperm-specific Phospholipase C zeta (PLCζ) is widely considered to be the sole, physiological stimulus responsible for the generation of Ca2+ oscillations that induce egg activation and early embryo development during ... -
Calmodulin Mutations Associated with Congenital Cardiac Disease Display Novel Biophysical and Biochemical Characteristics
( Elsevier , 2018 , Article)Calmodulin (CaM) is a cytoplasmic multifunctional calcium (Ca2+)-binding messenger that interacts with the cardiac ryanodine receptor (RyR2), a large transmembrane Ca2+ channel that mediates Ca2+ release from the sarcoplasmic ... -
Defective Interaction of Cam with RyR2 Cam-Binding Pocket Might Contribute to Arrhythmogenic Cardiac Disease
( Elsevier , 2020 , Article)Ryanodine receptor 2 (RyR2) is a large transmembrane calcium (Ca2+) release channel that mediates Ca2 release from the sarcoplasmic reticulum to activate cardiac muscle contraction. Calmodulin (CaM) regulation of RyR2 is ... -
Development of an in-house COVID-19 serology ELISA Test
( Hamad bin Khalifa University Press (HBKU Press) , 2021 , Article)Background: COVID-19 pandemic created an unprecedented demand for reagents and diagnostic tools to confirm COVID-19 cases. Thus, the development of a robust in-house diagnostic test is considered of high importance. Within ... -
Editorial: The role of calcium signaling in gametogenesis and early embryogenesis
( Frontiers Media , 2022 , Other)Calcium homeostasis exerts a profound role in determining the quality of gametogenesis, the resultant efficacy of fertilisation and ultimately the competency of embryogenesis. Perhaps no other single ion plays such a ... -
Essential Role of Sperm-Specific PLC-Zeta in Egg Activation and Male Factor Infertility: An Update.
( Frontiers Media , 2020 , Article Review)Sperm-specific phospholipase C zeta (PLCζ) is widely considered to be the physiological stimulus responsible for generating calcium (Ca) oscillations that induce egg activation and early embryonic development during mammalian ... -
Functional characterization of human myosin-binding protein C3 variants associated with hypertrophic cardiomyopathy reveals exon-specific cardiac phenotypes in zebrafish model
( Wiley , 2020 , Article)© 2020 Wiley Periodicals, Inc. Myosin-binding protein C 3 (MYBPC3) variants are the most common cause of hypertrophic cardiomyopathy (HCM). HCM is a complex cardiac disorder due to its significant genetic and clinical ... -
Health educators' professional agency in negotiating their Problem-Based Learning (PBL) facilitator roles: Q study.
( John Wiley & Sons, Ltd , 2022 , Article)University educators are expected to cope with emerging situations and complex issues in teaching and learning, and this requires them to be agentic and proactive. While professional agency of health educators has not been ... -
Increased de novo DNA methylation enzymes in sperm of individuals with varicocele
( pmc , 2021 , Article)Objective: Chronic genital heat-stress associated with varicocele leads to DNA hypo-methylation of spermatozoa. The objective of this study was comparing level of DNA methyl-transferases (DNMTs) in sperm of men suffering ... -
Life-threatening arrhythmogenic CaM mutations disrupt CaM binding to a distinct RyR2 CaM-binding pocket
( Elsevier , 2023 , Article)Calmodulin (CaM) modulates the activity of several proteins that play a key role in excitation-contraction coupling (ECC). In cardiac muscle, the major binding partner of CaM is the type-2 ryanodine receptor (RyR2) and ... -
Male infertility-linked point mutation reveals a vital binding role for the C2 domain of sperm PLC?
( Portland Press Ltd , 2017 , Article)Sperm-specific phospholipase C zeta (PLC?) is widely considered to be the physiological stimulus that evokes intracellular calcium (Ca2+) oscillations that are essential for the initiation of egg activation during mammalian ... -
Mutations in PLCδ1 associated with hereditary leukonychia display divergent PIP2 hydrolytic function
( Blackwell Publishing Ltd , 2016 , Article)Hereditary leukonychia is a rare genetic nail disorder characterized by distinctive whitening of the nail plate of all 20 nails. Hereditary leukonychia may exist as an isolated feature, or in simultaneous occurrence with ... -
Phospholipase C zeta profiles are indicative of optimal sperm parameters and fertilisation success in patients undergoing fertility treatment.
( wiley , 2020 , Article)Oocyte activation is driven by intracellular calcium (Ca ) oscillations induced by sperm-specific PLCζ, abrogation of which causes oocyte activation deficiency in humans. Clinical PLCζ investigations have been limited to ... -
Pyridoxine non-responsive p.R336C mutation alters the molecular properties of cystathionine beta-synthase leading to severe homocystinuria phenotype
( Elsevier , 2022 , Article)The prevalence of homocystinuria in Qatar is 1:1800, mainly due to a founder missense mutation p.R336C. • The cystathionine beta-synthase (CBS) R336C mutant was bacterially expressed, purified and its molecular properties ... -
SPERM FACTORS AND EGG ACTIVATION The structure and function relationship of sperm PLCZ1
( BioScientifica , 2022 , Article Review)In 2002, sperm-specific phospholipase C zeta1 (PLCZ1) was discovered and through these 20 years, it has been established as the predominant sperm oocyte-activating factor. PLCZ1 cRNA expression or direct protein microinjection ... -
The role and mechanism of action of sperm PLC-zeta in mammalian fertilisation
( Portland Press Ltd , 2017 , Article Review)At mammalian fertilisation, the fundamental stimulus that triggers oocyte (egg) activation and initiation of early embryonic development is an acute rise of the intracellular-free calcium (Ca2+) concentration inside the ...
