Browsing by Author "Lai, F. Anthony"
Now showing items 1-15 of 15
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Antigen Unmasking Is Required to Clinically Assess Levels and Localisation Patterns of Phospholipase C Zeta in Human Sperm
Kashir, Junaid; Mistry, Bhavesh V.; BuSaleh, Lujain; Nomikos, Michail; Almuqayyil, Sarah; Abu-Dawud, Raed; AlYacoub, Nadya; Hamdan, Hamdan; AlHassan, Saad; Lai, F. Anthony; Assiri, Abdullah M.; Coskun, Serdar... more authors ... less authors ( MDPI , 2023 , Article)Mammalian oocyte activation is initiated by intracellular calcium (Ca2+) oscillations, driven by the testis-specific phospholipase C zeta (PLCζ). Sperm PLCζ analysis represents a diagnostic measure of sperm fertilisation ... -
Arrhythmogenic calmodulin E105A mutation alters cardiac RyR2 regulation leading to cardiac dysfunction in zebrafish
Da'as, Sahar I; Thanassoulas, Angelos; Calver, Brian L; Beck, Konrad; Salem, Rola; Saleh, Alaaeldin; Kontogianni, Iris; Al-Maraghi, Ali; Nasrallah, Gheyath K; Safieh-Garabedian, Bared; Toft, Egon; Nounesis, George; Lai, F Anthony; Nomikos, Michail... more authors ... less authors ( Wiley , 2019 , Article)Calmodulin (CaM) is a universal calcium (Ca )-binding messenger that regulates many vital cellular events. In cardiac muscle, CaM associates with ryanodine receptor 2 (RyR2) and regulates excitation-contraction coupling. ... -
The Arrhythmogenic E105A CAM Mutation Dysregulates Normal Cardiac Function in Zebrafish by Altering CAM-Ca2+ and CAM-RyR2 Interactions
Nomikos, Michail; I. Da’as, Sahar; Thanassoulas, Angelos; Salem, Rola; L. Calver, Brian; Saleh, Alaaeldin; Al-Maraghi, Ali; K. Nasrallah, Gheyath; Safieh-Garabedian, Bared; Toft, Egon; Nounesis, George; Lai, F. Anthony... more authors ... less authors ( Elsevier , 2019 , Article)Calmodulin (CaM) is a multifunctional calcium (Ca2+)-binding messenger that directly interacts with the cardiac ryanodine receptor 2 (RyR2), a large transmembrane Ca2+ channel that mediates Ca2+ release from the sarcoplasmic ... -
Calmodulin Interacts and Regulates Enzyme Activity of the Mammalian Sperm Phospholipase C
Nomikos, Michail; Thanassoulas, Angelos; L. Calver, Brian; Theodoridou, Maria; Buntwal, Luke; Sideratou, Zili; Nounesis, George; Lai, F. Anthony... more authors ... less authors ( Elsevier , 2017 , Article)Sperm-specific Phospholipase C zeta (PLCζ) is widely considered to be the sole, physiological stimulus responsible for the generation of Ca2+ oscillations that induce egg activation and early embryo development during ... -
Defective Interaction of Cam with RyR2 Cam-Binding Pocket Might Contribute to Arrhythmogenic Cardiac Disease
Nomikos, Michail; Thanassoulas, Angelos; Vassilakopoulou, Vyronia; L. Calver, Brian; Livaniou, Evangelia; Safieh-Garabedian, Bared; Toft, Egon; Nounesis, George; Lai, F. Anthony... more authors ... less authors ( Elsevier , 2020 , Article)Ryanodine receptor 2 (RyR2) is a large transmembrane calcium (Ca2+) release channel that mediates Ca2 release from the sarcoplasmic reticulum to activate cardiac muscle contraction. Calmodulin (CaM) regulation of RyR2 is ... -
Editorial: The role of calcium signaling in gametogenesis and early embryogenesis
Kashir, Junaid; Lai, F. Anthony; Nomikos, Michail ( Frontiers Media , 2022 , Other)Calcium homeostasis exerts a profound role in determining the quality of gametogenesis, the resultant efficacy of fertilisation and ultimately the competency of embryogenesis. Perhaps no other single ion plays such a ... -
Essential Role of Sperm-Specific PLC-Zeta in Egg Activation and Male Factor Infertility: An Update.
Saleh, Alaaeldin; Kashir, Junaid; Thanassoulas, Angelos; Safieh-Garabedian, Bared; Lai, F Anthony; Nomikos, Michail... more authors ... less authors ( Frontiers Media , 2020 , Article Review)Sperm-specific phospholipase C zeta (PLCζ) is widely considered to be the physiological stimulus responsible for generating calcium (Ca) oscillations that induce egg activation and early embryonic development during mammalian ... -
Identification of an amino-terminus determinant critical for ryanodine receptor/Ca2+ release channel function.
Seidel, Monika; de Meritens, Camille Rabesahala; Johnson, Louisa; Parthimos, Dimitris; Bannister, Mark; Thomas, N Lowri; Ozekhome-Mike, Esizaze; Lai, F Anthony; Zissimopoulos, Spyros... more authors ... less authors ( Oxford University Press , 2020 , Article)The cardiac ryanodine receptor (RyR2), which mediates intracellular Ca2+ release to trigger cardiomyocyte contraction, participates in development of acquired and inherited arrhythmogenic cardiac disease. This study was ... -
Impaired Binding to Junctophilin-2 and Nanostructural Alteration in CPVT Mutation
Yin, Liheng; Jr, Alexandra Zahradnikova; Rizzetto, Riccardo; Boncompagni, Simona; Meritens, Camille Rabesahala de; Zhang, Yadan; Joanne, Pierre; Marqués-Sulé, Elena; Aguilar-Sánchez, Yuriana; Fernández-Tenorio, Miguel; Villejoubert, Olivier; Li, Linwei; Wang, Yue Yi; Mateo, Philippe; Nicolas, Valérie; Gerbaud, Pascale; Lai, F. Anthony; Perrier, Romain; Álvarez, Julio L.; Niggli, Ernst; Valdivia, Héctor H.; Valdivia, Carmen R.; Ramos-Franco, Josefina; Zorio, Esther; Zissimopoulos, Spyros; Protasi, Feliciano; Benitah, Jean-Pierre; Gómez, Ana M.... more authors ... less authors ( American Heart Association , 2021 , Article)RATIONALE: Catecholaminergic polymorphic ventricular tachycardia is a rare disease, manifested by syncope or sudden death in children or young adults under stress conditions. Mutations in the Ca2+ release channel/type 2 ... -
Life-threatening arrhythmogenic CaM mutations disrupt CaM binding to a distinct RyR2 CaM-binding pocket
Angelos, Thanassoulas; Vassilakopoulou, Vyronia; Calver, Brian L.; Buntwal, Luke; Smith, Adrian; Lai, Christopher; Kontogianni, Iris; Livaniou, Evangelia; Nounesis, George; Lai, F. Anthony; Nomikos, Michail... more authors ... less authors ( Elsevier , 2023 , Article)Calmodulin (CaM) modulates the activity of several proteins that play a key role in excitation-contraction coupling (ECC). In cardiac muscle, the major binding partner of CaM is the type-2 ryanodine receptor (RyR2) and ... -
Male infertility-linked point mutation reveals a vital binding role for the C2 domain of sperm PLC?
Nomikos, Michail; Stamatiadis, Panagiotis; Sanders, Jessica R.; Beck, Konrad; Calver, Brian L.; Buntwal, Luke; Lofty, Morgan; Sideratou, Zili; Swann, Karl; Lai, F. Anthony... more authors ... less authors ( Portland Press Ltd , 2017 , Article)Sperm-specific phospholipase C zeta (PLC?) is widely considered to be the physiological stimulus that evokes intracellular calcium (Ca2+) oscillations that are essential for the initiation of egg activation during mammalian ... -
Mutations in PLCδ1 associated with hereditary leukonychia display divergent PIP2 hydrolytic function
Nomikos, Michail; Thanassoulas, Angelos; Beck, Konrad; Theodoridou, Maria; Kew, Jasmine; Kashir, Junaid; Calver, Brian L.; Matthews , Emily; Rizkallah, Pierre; Sideratou, Zili; Nounesis, George; Lai, F. Anthony... more authors ... less authors ( Blackwell Publishing Ltd , 2016 , Article)Hereditary leukonychia is a rare genetic nail disorder characterized by distinctive whitening of the nail plate of all 20 nails. Hereditary leukonychia may exist as an isolated feature, or in simultaneous occurrence with ... -
Phospholipase C zeta profiles are indicative of optimal sperm parameters and fertilisation success in patients undergoing fertility treatment.
Kashir, Junaid; Mistry, Bhavesh V; BuSaleh, Lujain; Abu-Dawas, Reema; Nomikos, Michail; Ajlan, Ahmed; Abu-Dawud, Raed; AlYacoub, Nadya; AlHassan, Saad; Lai, F Anthony; Assiri, Abdullah M; Coskun, Serdar... more authors ... less authors ( wiley , 2020 , Article)Oocyte activation is driven by intracellular calcium (Ca ) oscillations induced by sperm-specific PLCζ, abrogation of which causes oocyte activation deficiency in humans. Clinical PLCζ investigations have been limited to ... -
SPERM FACTORS AND EGG ACTIVATION The structure and function relationship of sperm PLCZ1
Thanassoulas, Angelos; Swann, Karl; Lai, F. Anthony; Nomikos, Michail ( BioScientifica , 2022 , Article Review)In 2002, sperm-specific phospholipase C zeta1 (PLCZ1) was discovered and through these 20 years, it has been established as the predominant sperm oocyte-activating factor. PLCZ1 cRNA expression or direct protein microinjection ... -
The role and mechanism of action of sperm PLC-zeta in mammalian fertilisation
Nomikos, Michail; Kashir, Junaid; Lai, F. Anthony ( Portland Press Ltd , 2017 , Article Review)At mammalian fertilisation, the fundamental stimulus that triggers oocyte (egg) activation and initiation of early embryonic development is an acute rise of the intracellular-free calcium (Ca2+) concentration inside the ...