تصفح حسب الموضوع "Bacillus thuringiensis"
السجلات المعروضة 1 -- 20 من 26
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Agrotis segetum midgut putative receptor of Bacillus thuringiensis vegetative insecticidal protein Vip3Aa16 differs from that of Cry1Ac toxin
( Elsevier Inc. , 2013 , Article)Considering the fact that Agrotis segetum is one of the most pathogenic insects to vegetables and cereals in the world, particularly in Africa, the mode of action of Vip3Aa16 of Bacillus thuringiensis BUPM95 and Cry1Ac of ... -
Amino acids Y229 and F603 are involved in Bacillus thuringiensis Cry1Ac delta-endotoxin stability and toxicity
( Blackwell Publishing , 2012 , Article)Bacillus thuringiensis Cry1Ac toxin shares structurally five conserved blocs with the other δ-endotoxins. To study the role of some amino acids belonging to these regions, two mutations, Y(229) P and F(603) S, were introduced ... -
Application of scanning electron microscopy and MALDI-TOF MS in the exploration of the parasporal insecticidal crystals of Bacillus thuringiensis strains isolated from Qatar soil
( Elsevier Ltd , 2022 , Article)Bacillus thuringiensis (Bt) plays an important role in health and agriculture due to its insecticidal activities. In this study, six Bt strains isolated from Qatar soil were used to explore their crystals morphologies and ... -
Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity
( Academic Press , 2021 , Article)Bacillus thuringiensis subsp. israelensis (Bti) spherical parasporal crystal contains several insecticidal proteins used as environmentally safe alternative to toxic chemical pesticides. The exploration of a Bti strain ... -
Characterisation of novel Bacillus thuringiensis isolates against Aedes aegypti (Diptera: Culicidae) and Ceratitis capitata (Diptera:Tephridae)
( Elsevier Inc. , 2015 , Article)Bacillus thuringiensis is successfully used in pest management strategies as an eco-friendly bioinsecticide. Isolation and identification of new strains with a wide variety of target pests is an ever growing field. In this ... -
Characterization of tunisian Bacillus thuringiensis strains with abundance of kurstaki subspecies harbouring insecticidal activities against the lepidopteran insect ephestia kuehniella
( Springer US , 2010 , Article)The study of 257 crystal-producing Bacillus thuringiensis isolates from bioinsecticide free soil samples collected from different sites in Tunisia, was performed by PCR amplification, using six primer pairs specific for ... -
Combinatorial effect of Bacillus thuringiensis kurstaki and Photorhabdus luminescens against Spodoptera littoralis (Lepidoptera: Noctuidae).
( WILEY-VCH , 2014 , Article)Spodoptera littoralis, one of the major pests of many important crop plants, is more susceptible to Bacillus thuringiensis aizawai delta-endotoxins than to those of Bacillus thuringiensis kurstaki. Within the framework of ... -
Comparative study of Bacillus thuringiensis Cry1Ia and Cry1Aa delta-endotoxins : activation process and toxicity against Prays oleae
( Elsevier Inc. , 2010 , Article)Cry1Ia and Cry1Aa proteins exhibited toxicities against Prays oleae with LC(50) of 189 and 116 ng/cm(2), respectively. The ability to process Cry1Ia11 protoxin by trypsin, chymotrypsin and P. oleae larvae proteases was ... -
Construction of a Bacillus thuringiensis genetically-engineered strain harbouring the secreted Cry1Ia delta-endotoxin in its crystal
( Springer Netherlands , 2011 , Article)Unlike other Bacillus thuringiensis Cry proteins, Cry1 Ia does not form a crystal since it is a secreted delta-endotoxin. We have engineered a Cry1 Iac chimeric protein by substituting the C-terminal part of Cry1 Ia by the ... -
Correlation between delta-endotoxin and proteolytic activities produced by Bacillus thuringiensis var. kurstaki growing in an economic production medium
( Taylor & Francis , 2013 , Article)Bacillus thuringiensis is a Gram positive bacterium that produces an insecticidal crystalline protein making it one of the most important biocontrol agents for pest management. Bioinsecticides based on B. thuringiensis ... -
Diversity of Bacillus thuringiensis Strains From Qatar as Shown by Crystal Morphology, d-Endotoxins and Cry Gene Content
( Frontiers Media , 2018 , Article)Bacillus thuringiensis (Bt) based insecticidal formulations have been recognized as one of the most successful, environmentally safe and sustainable method of controlling insect pests. Research teams worldwide are in ... -
Evidence of the Involvement of E358, A498 and C571 of a New Cry1Ac δ-endotoxin of Bacillus thuringiensis in its High Insecticidal Activity Against Ephestia kuehniella
( Humana Press Inc , 2010 , Article)A new cry1Ac-type gene was cloned from Bacillus thuringiensis strain BLB1, sequenced and expressed. The deduced amino acid sequence of the polypeptide has a predicted molecular mass of 132.186 kDa. The amino acid sequence ... -
Histopathological effects and determination of the putative receptor of Bacillus thuringiensis Cry1Da toxin in Spodoptera littoralis midgut
( Elsevier , 2012 , Article)Bacillus thuringiensis subsp. aizawai strain HD133, known by its effectiveness against Spodoptera species, produces many insecticidal proteins including Cry1Ab, Cry1Ca and Cry1Da. In the present study, the insecticidal ... -
Improvement of Bacillus thuringiensis bioinsecticide production by sporeless and sporulating strains using response surface methodology
( Elsevier B.V. , 2011 , Article)Statistical experimental designs, involving a Plackett-Burman design followed by a rotatable central composite design were used to optimize the culture medium constituents for Bacillus thuringiensis bioinsecticide production. ... -
Investigation of Bacillus Thuringiensis Plasmid Instability and its Effect on the Synthesis of Crystals
( Qatar University Press , 2020 , Poster)In order to explore plasmid instability in Bt, four Bt strains belonging to two Bt subspecies were cultured at 42°C for 9 days. HD1 and QBT376 belong to subspecies kurstaki, while H14 and QBT218 belong to subspecies ... -
Investigation of novel bacteriocin producers of Bacillus thuringiensis and partial characterization of two new bacteriocins: Thuricin 466 and thuricin 4Q7
( Elsevier Ltd , 2024 , Article)This study assessed the bacteriocin-producing capability of 140 strains of Bacillus thuringiensis isolated from Qatar. Numerous Bt strains exhibited strong antibacterial activity against both the foodborne pathogen B. ... -
Multiple linear regression and artificial neural networks for delta-endotoxin and protease yields modelling of Bacillus thuringiensis
( Springer Verlag , 2017 , Article)The aim of the present work was to develop a model that supplies accurate predictions of the yields of delta-endotoxins and proteases produced by B. thuringiensis var. kurstaki HD-1. Using available medium ingredients as ... -
Overproduction of the Bacillus thuringiensis Vip3Aa16 toxin and study of its insecticidal activity against the carob moth Ectomyelois ceratoniae
( Elsevier , 2015 , Article)The vip3Aa16 gene of Bacillus thuringiensis strain BUPM95 was cloned and expressed in Escherichia coli. Optimization of Vip3A16 protein expression was conducted using Plackett–Burman design and response surface methodology. ... -
Production of Polyclonal and Monoclonal Antibodies Against the Bacillus thuringiensis Vegetative Insecticidal Protein Vip3Aa16
( Springer International Publishing AG , 2014 , Article)The aim of this study is to establish a quantitative determination of the vegetative insecticidal protein Vip3A from the culture supernatant of Bacillus thuringiensis either by ELISA or by the conventional quantification ... -
Purification and characterization of a new Bacillus thuringiensis bacteriocin active against Listeria monocytogenes, Bacillus cereus and Agrobacterium tumefaciens
( Springer Science , 2011 , Article)This study reports on the identification, characterization and purification of a new bacteriocin, named Bacthuricin F103, from a Bacillus thuringiensis strain BUPM103. Bacthuricin F103 production began in the early exponential ...