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Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar
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Date
2021-11
Author
R. Hasan, Mohammad, K.R. Kalikiri, Mahesh, Mirza, Faheem, Sundararaju, Sathyavathi, Sharma, Anju, Xaba, Thabisile, Lorenz, Stephan, Chemaitelly, Hiam, A. El-Kahlout, Reham, Ming Tsui, Kin, M. Yassine, Hadi, V. Coyle, Peter, Al Khal, Abdullatif, Bertollini, Roberto, H. Al Thani, Mohamed, J. Abu-Raddad, Laith, Tang, Patrick
Abstract
Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully genotyped, revealing the importation and transmission dynamics of these three variants in Qatar.