3D Interconnected Mesoporous Alumina with Loaded Hemoglobin as a Highly Active Electrochemical Biosensor for H<inf>2</inf>O<inf>2</inf>
Author | Yang, X. |
Author | Yang, Xuanyu |
Author | Cheng, Xiaowei |
Author | Song, Hongyuan |
Author | Ma, Junhao |
Author | Pan, Panpan |
Author | Elzatahry, Ahmed A. |
Author | Su, Jiacan |
Author | Deng, Yonghui |
Available date | 2019-09-17T10:26:46Z |
Publication Date | 2018-06-06 |
Publication Name | Advanced Healthcare Materials |
Identifier | http://dx.doi.org/10.1002/adhm.201800149 |
Citation | Yang, X., Cheng, X., Song, H., Ma, J., Pan, P., Elzatahry, A. A., Su, J., Deng, Y., Adv. Healthcare Mater. 2018, 7, 1800149. https://doi.org/10.1002/adhm.201800149 |
ISSN | 2192-2640 |
Abstract | © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Alumina is one of the most common and stable metal oxides in nature, which has been developed as a novel adsorbent in enrichment of biomolecules due to its excellent affinity to phosphor or amino groups. In this study, ordered mesoporous alumina (OMA) with interconnected mesopores and surface acidic property is synthesized through a solvent evaporation induced co-assembly process using poly(ethylene oxide)-b-polystyrene (PEO-b-PS) diblock copolymer as a template and aluminium acetylacetonate (Al(acac)3) as the aluminium source. The pore size (12.1–19.7 nm), pore window size (3.5–9.0 nm) and surface acidity (0.092–0.165 mmol g−1) can be precisely adjusted. The highly porous structure endows the OMA materials with high hemoglobin (Hb) immobilization capacity (170 mg g−1). The obtained Hb@OMA composite is used as an electrocatalyst of biosensor for convienet and fast detection of hydrogen peroxide (H2O2) with a low H2O2 detection limit of 1.7 × 10−8 m and a wide linear range of 2.5 × 10−8 to 5.0 × 10−5 m. Moreover, the Hb@OMA sensors show a good performance in real time detection of H2O2 released from Homo sapiens bone osteosarcoma, indicating their potential application in complex biological processes. |
Sponsor | This work was supported by the NSF of China (51372041, 51422202, 21673048, 91749204 and 81771491), Shanghai Pujiang Program, China (No. 16PJ1401100), Key Basic Research Program of Science and Technology Commission of Shanghai Municipality (17JC1400100), Shanghai Municipal Science and Technology Commission Key Program (15411950600), Youth Top-notch Talent Support Program of China, the state key laboratory of Transducer Technology of China (Grant No. SKT1503), the Outstanding Talents Cultivation Program of Shanghai Health System (2017BR011), and Qatar University under GCC Co-Fund Program, Grant GCC-2017-001. The authors thank Prof. Guangrong Zhou for assistance in TEM characterization. |
Language | en |
Publisher | Wiley-VCH Verlag |
Subject | biosensors electrocatalysts hemoglobin hydrogen peroxide immobilization porous alumina |
Type | Article |
Issue Number | 11 |
Volume Number | 7 |
ESSN | 2192-2659 |
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