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AuthorChrismas B.
AuthorTaylor L.
AuthorSmith A.
AuthorPemberton P.
AuthorSiegler J.C.
AuthorMidgley A.W.
Available date2020-03-18T08:10:11Z
Publication Date2018
Publication NameMeasurement in Physical Education and Exercise Science
ResourceScopus
ISSN1091367X
URIhttp://dx.doi.org/10.1080/1091367X.2017.1412317
URIhttp://hdl.handle.net/10576/13370
AbstractTo examine the reproducibility of three measurement techniques used to determine creatine kinase, interleukin-6 and high-sensitivity C-reactive protein, 50 participants had blood samples taken on two occasions. Fingertip plasma samples were analysed using the Reflotron for CK determination. Venous blood samples collected into serum separator tubes were used for IL-6 and hs-CRP analyses. IL-6 was measured using an enzyme linked immune assay development kit. The hs-CRP was measured by an in-house ELISA method. Dependent t-tests showed no systematic bias between samples. The interdian CV was 20.0% for CK, 15.3% for IL-6 and 44.2% for hs-CRP. The intraclass correlation coefficient was 0.90 for CK, 0.98 for IL-6 and 0.70 for hs-CRP. The 95% limits of agreement were ?69.7 to 63.5 IU/L for CK, ?1.48 to 1.80?pg/ml for IL-6 and ?1.10 to 0.91??g/L for hs-CRP. The results demonstrate low absolute reproducibility, which may obscure a true experimental effect. ? 2018, ? 2018 Taylor & Francis.
SponsorAssociation of Anaesthetists of Great Britain and Ireland, University of Western Sydney, Aspetar Orthopaedic and Sports Medicine Hospital, Central Manchester University Hospitals NHS Foundation Trust, Loughborough University, College of Arts and Sciences, Boston University, University of Hull
Languageen
PublisherRoutledge
Subjectbiomarkers
cytokines
Exercise induced muscle damage
inflammation
reliability
TitleReproducibility of measurement techniques used for creatine kinase, interleukin-6 and high-sensitivity C-reactive protein determination over a 48 h period in males and females
TypeArticle
Pagination191 - 199
Issue Number3
Volume Number22
dc.accessType Abstract Only


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