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AuthorMohamed Atia, Fathy Atia
AuthorAhmadi, Ahmad Ali
AuthorAlSafran, Mohammed
Available date2020-10-22T07:00:23Z
Publication Date2020
Publication NameQatar University Annual Research Forum & Exhibition 2020
CitationMohamedAtia F.A., Ahmadi A.A., AlSafran M., "method validation of drug quantification poster"", Qatar University Annual Research Forum and Exhibition (QUARFE 2020), Doha, 2020, https://doi.org/10.29117/quarfe.2020.0058
URIhttps://doi.org/10.29117/quarfe.2020.0058
URIhttp://hdl.handle.net/10576/16593
AbstractA lowest detection limit with straight linearity were obtained by developing a method to analyze Phenacetin (Phe) in both aqueous and organic extraction by using Liquid chromatography Triple Quoadrpole mass with electrospray ionization (LCMSMS/ESI). The validation of the developed method was carried out according to ICH Harmonized Tripartite guideline. Validation criteria obtained were; the method detection limit MDL is 0.089 ng/ml, method quantification limit MQL is 0.19 ng/ml while the calibration curve linear from 0.1 to 1000 ng/mL with correlation coefficient R2 is 0.9994, Accuracy and precision up to 97% and the repeatability inter and intraday for six replicates of three concentration with RSD 2.1%. Separation occurred using Nova Pack C18 4 um, 150 x 3.9 mm column, using acetonitrile : 0.1% Formic acid 60:40% (v:v) at flow rate 1ml/min. the detector was triple quad mass spectrometry at multi-reaction mode MRM to detect parent mass 180.1 at frag 97 to transition fragments 110 and 138 at collision voltages 16 and 12 respectively.
Languageen
PublisherQatar University Press
SubjectMethod validation; Phenacetin; LCMSMS
Titlemethod validation of drug quantification poster
TypePoster


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