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AuthorAl-Qeraiwi, Maha
AuthorAl-Rashid, Manar
AuthorRizk, Nasser
AuthorEl Gamal, Abdelrahman
AuthorFadl, Amena
Available date2020-10-26T09:32:39Z
Publication Date2020
Publication NameQatar University Annual Research an Exhibition 2020 (quarfe)
CitationAlQeraiwi M., AlRashid M., Rizk N., ElGamal A., Fadl A., " Hepatic Gene Expression Profile of Lipid Metabolism of Obese Mice After Treatment with AntiObesity Drug", Qatar University Annual Research Forum and Exhibition (QUARFE 2020), Doha, 2020, https://doi.org/10.29117/quarfe.2020.0214
URIhttps://doi.org/10.29117/quarfe.2020.0214
URIhttp://hdl.handle.net/10576/16797
AbstractObesity is a global disorder with multifactorial causes. The liver plays a vital role in fat metabolism. Disorder of hepatic fat metabolism is associated with obesity and causes fatty liver. High fat diet intake (HFD) to mice causes the development of dietinduced obesity (DIO). The study aimed to detect the effects of anti-obesity drugs (sulforaphane; SFN and leptin) on hepatic gene expression of fat metabolism in mice that were fed HFD during an early time of DIO. Twenty wild types (WT) CD1 male mice aged ten weeks were fed a high fat diet. The mice were treated with vehicle; Veh (control group), and SFN, then each group is treated with leptin or saline. Four groups of treatment were: control group (vehicle + saline), Group 2 (vehicle + leptin), group 3 (SFN + saline), and group 4 (SFN + leptin). Body weight and food intake were monitored during the treatment period. Following the treatments of leptin 24 hour, fasting blood samples and liver tissue was collected, and Total RNA was extracted then used to assess the gene expression of 84 genes involved in hepatic fat metabolism using RT-PCR profiler array technique. leptin treatment upregulated fatty acid betaoxidation (Acsbg2, Acsm4) and fatty acyl-CoA biosynthesis (Acot6, Acsl6), and down-regulated is fatty acid transport (Slc27a2). SFN upregulated acylCoA hydrolase (Acot3) and long chain fatty acid activation for lipids synthesis and beta oxidation (Acsl1). leptin + SFN upregulated fatty acid beta oxidation (Acad11, Acam) and acyl-CoA hydrolase (Acot3, Acot7), and downregulated fatty acid elongation (Acot2). As a result, treatment of both SFN and leptin has more profound effects on ameliorating pathways involved in hepatic lipogenesis and TG accumulation and lipid profile of TG and TC than other types of intervention. We conclude that early intervention of obesity pa could ameliorate the metabolic changes of fat metabolism in liver as observed in WT mice on HFD in response to anti-obesity treatment
Languageen
PublisherQatar University Press
SubjectGene Expression, Lipid, Obese
TitleHepatic Gene Expression Profile of Lipid Metabolism of Obese Mice After Treatment with AntiObesity Drug
TypePoster
dc.accessType Open Access


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