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المؤلفTuttle, James A.
المؤلفChrismas, Bryna C. R.
المؤلفGibson, Oliver R.
المؤلفBarrington, James H.
المؤلفHughes, David C.
المؤلفCastle, Paul C.
المؤلفMetcalfe, Alan J.
المؤلفMidgley, Adrian W.
المؤلفPearce, Oliver
المؤلفKabir, Chindu
المؤلفRayanmarakar, Faizal
المؤلفAl-Ali, Sami
المؤلفLewis, Mark P.
المؤلفTaylor, Lee
تاريخ الإتاحة2021-01-27T11:06:53Z
تاريخ النشر2017
اسم المنشورFrontiers in Physiology
المصدرScopus
الرقم المعياري الدولي للكتاب1664042X
معرّف المصادر الموحدhttp://dx.doi.org/10.3389/fphys.2017.00473
معرّف المصادر الموحدhttp://hdl.handle.net/10576/17469
الملخصThe leukocyte heat shock response (HSR) is used to determine individual's thermotolerance. The HSR and thermotolerance are enhanced following interventions such as preconditioning and/or acclimation/acclimatization. However, it is unclear whether the leukocyte HSR is an appropriate surrogate for the HSR in other tissues implicated within the pathophysiology of exertional heat illnesses (e.g., skeletal muscle), and whether an acute preconditioning strategy (e.g., downhill running) can improve subsequent thermotolerance. Physically active, non-heat acclimated participants were split into two groups to investigate the benefits of hot downhill running as preconditioning strategy. A hot preconditioning group (HPC; n = 6) completed two trials (HPC1HOTDOWN and HPC2HOTDOWN) of 30 min running at lactate threshold (LT) on −10% gradient in 30°C and 50% relative humidity (RH) separated by 7 d. A temperate preconditioning group (TPC; n = 5) completed 30 min running at LT on a −1% gradient in 20°C and 50% (TPC1TEMPFLAT) and 7 d later completed 30 min running at LT on −10% gradient in 30°C and 50% RH (TPC2HOTDOWN). Venous blood samples and muscle biopsies (vastus lateralis; VL) were obtained before, immediately after, 3, 24, and 48 h after each trial. Leukocyte and VL Hsp72, Hsp90α, and Grp78 mRNA relative expression was determined via RT-QPCR. Attenuated leukocyte and VL Hsp72 (2.8 to 1.8 fold and 5.9 to 2.4 fold; p < 0.05) and Hsp90α mRNA (2.9 to 2.4 fold and 5.2 to 2.4 fold; p < 0.05) responses accompanied reductions (p < 0.05) in physiological strain [exercising rectal temperature (−0.3°C) and perceived muscle soreness (~ −14%)] during HPC2HOTDOWN compared to HPC1HOTDOWN (i.e., a preconditioning effect). Both VL and leukocyte Hsp72 and Hsp90α mRNA increased (p < 0.05) simultaneously following downhill runs and demonstrated a strong relationship (p < 0.01) of similar magnitudes with one another. Hot downhill running is an effective preconditioning strategy which ameliorates physiological strain, soreness and Hsp72 and Hsp90α mRNA responses to a subsequent bout. Leukocyte and VL analyses are appropriate tissues to infer the extent to which the HSR has been augmented.
اللغةen
الناشرFrontiers Media S.A.
الموضوعCross tolerance
Downhill running
Heat shock response
Heat stress
Heat tolerance
Preconditioning
Thermotolerance
العنوانThe Hsp72 and Hsp90a mRNA responses to hot downhill running are reduced following a prior bout of hot downhill running, and occur concurrently within leukocytes and the vastus lateralis
النوعArticle
رقم العددJUL
رقم المجلد8
dc.accessType Open Access


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