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AuthorYuan, Heyang
AuthorMiller, Jennifer H.
AuthorAbu-Reesh, Ibrahim M.
AuthorPruden, Amy
AuthorHe, Zhen
Available date2021-04-22T13:00:29Z
Publication Date2016
Publication NameScience of the Total Environment
ResourceScopus
URIhttp://dx.doi.org/10.1016/j.scitotenv.2016.07.002
URIhttp://hdl.handle.net/10576/18322
Abstractnaerobic biotechnologies can effectively remove antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs), but there is a need to better understand the mechanisms. Here we employ bioelectrochemical systems (BES) as a platform to investigate the fate of a native tetracycline and sulfonamide-resistant Escherichia coli strain and its ARGs. The E. coli strain carrying intI1, sulI and tet(E) was isolated from domestic wastewater and dosed into a tubular BES. The BES was first operated as a microbial fuel cell (MFC), with aeration in the cathode, which resulted in enhanced removal of E. coli and ARGs by ~ 2 log (i.e., order of magnitude) when switched from high current to open circuit operation mode. The BES was then operated as a microbial electrolysis cell (MEC) to exclude the effects of oxygen diffusion, and the removal of E. coli and ARGs during the open circuit configuration was again 1–2 log higher than that at high current mode. Significant correlations of E. coli vs. current (R2 = 0.73) and ARGs vs. E. coli (R2 ranged from 0.54 to 0.87), and the fact that the BES substrate contained no electron acceptors, implied that the persistence of the E. coli and its ARGs was determined by the availability of indigenous electron acceptors in the BES, i.e., the anode electrode or the electron shuttles generated by the exoelectrogens. Subsequent experiments with pure-culture tetracycline and sulfonamide-resistant E. coli being incubated in a two-chamber MEC and serum bottles demonstrated that the E. coli could survive by respiring anode electrode and/or electron shuttles released by exoelectrogens, and ARGs persisted with their host E. coli.
SponsorThe authors thank Dr. Husen Zhang at Leidos Biomedical Research, Inc. for the assistance with qPCR. This work was made possible by NPRP grant # 6-289-2-125 from the Qatar National Research Fund (a member of Qatar Foundation). The statements made herein are solely the responsibility of the authors.
Languageen
PublisherElsevier B.V.
SubjectAnodes
Antibiotics
Bacteria
Electrodes
Electrons
Escherichia coli
Fuel cells
Genes
Reconfigurable hardware
Regenerative fuel cells
Sulfur compounds
Anaerobic conditions
Antibiotic resistance genes
Antibiotic-resistant bacteria
Antibiotic-resistant escherichia coli
Antibiotic-resistant genes
Bio-electrochemical systems
Bioelectrochemical systems (BES)
Electron acceptor
Microbial fuel cells
electron acceptor
oxidizing agent
sulfonamide
tetracycline
unclassified drug
antiinfective agent
sulfonamide
tetracycline derivative
anoxic conditions
antibiotic resistance
biotechnology
electrochemistry
electron
fecal coliform
wastewater
wastewater treatment
aeration
anaerobic reactor
anode
antibiotic resistance
Article
bacterial gene
bacterial survival
bacterium culture
bacterium isolation
bioelectrochemical system
cathode
controlled study
correlation analysis
electric current
electrical equipment
electrode
Escherichia coli
exoelectrogen
integron
intI1 gene
microbial electrolysis cell
microbial fuel cell
nonhuman
oxygen diffusion
priority journal
proton transport
sulI gene
tet gene
waste water
waste water management
anaerobic growth
electron
Escherichia coli
genetics
integron
microbiology
physiology
sewage
tetracycline resistance
Escherichia coli
Anaerobiosis
Anti-Bacterial Agents
Drug Resistance, Bacterial
Electrons
Escherichia coli
Genes, Bacterial
Integrons
Sulfonamides
Tetracycline Resistance
Tetracyclines
Waste Disposal, Fluid
Waste Water
TitleEffects of electron acceptors on removal of antibiotic resistant Escherichia coli, resistance genes and class 1 integrons under anaerobic conditions
TypeArticle
Pagination1587-1594
Volume Number569-570
dc.accessType Abstract Only


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