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المؤلفMarei , Hany
المؤلفAlthani , Asma
المؤلفAfifi , Nahla
المؤلفMichetti , Fabricio
المؤلفPescatori , Mario
المؤلفPallini , Roberto
المؤلفCasalbore , Patricia
المؤلفCenciarelli , Carlo
المؤلفSchwartz , Philip
المؤلفAhmed , Abd-Elmaksoud
تاريخ الإتاحة2022-03-31T08:51:58Z
تاريخ النشر2012
اسم المنشورThe Qatar International Conference on Stem Cell Science and Policy
المصدرqscience
الاقتباسMarei , Hany., Althani , Asma., Afifi , Nahla., Michetti , Fabricio., Pescatori , Mario., Pallini , Roberto., Casalbore , Patricia., Cenciarelli , Carlo., Schwartz , Philip., Ahmed , Abd-Elmaksoud. (2012). Gene Expression Profiling of Embryonic Human Neural Stem Cells and Dopaminergic Neurons from Adult Human Substantia Nigra. The Qatar International Conference on Stem Cell Science and Policy: http://dx.doi.org/10.5339/qproc.2012.stem.1.56.
الرقم المعياري الدولي للكتاب2226-9649
معرّف المصادر الموحدhttps://doi.org/10.5339/qproc.2012.stem.1.56
معرّف المصادر الموحدhttp://hdl.handle.net/10576/29227
الملخصNeural stem cells (NSC) with self-renewal and multipotent properties serve as an ideal cell source for transplantation to treat neurodegenerative insults such as Parkinson's disease. We used Agilent's and Illumina Whole Human Genome Oligonucleotide Microarray to compare the genomic profiles of human embryonic NSC at a single time point in culture, and a multicellular tissue from postmortem adult substantia nigra (SN) which are rich in dopaminergic (DA) neurons. We identified 13525 up-regulated genes in both cell types of which 3737 (27.6%) genes were up-regulated in the hENSC, 4116 (30.4%) genes were up-regulated in the human substantia nigra dopaminergic cells, and 5672 (41.93%) were significantly up-regulated in both cell population. Careful analysis of the data that emerged using DAVID has permitted us to distinguish several genes and pathways that are involved in dopaminergic (DA) differentiation, and to identify the crucial signaling pathways that direct the process of differentiation. The set of genes expressed more highly at hENSC is enriched in molecules known or predicted to be involved in the M phase of the mitotic cell cycle. On the other hand, the genes enriched in SN cells include a different set of functional categories, namely synaptic transmission, central nervous system development, structural constituents of the myelin sheath, the internode region of axons, myelination, cell projection, cell somata, ion transport, and the voltage-gated ion channel complex. Our results were also compared with data from various databases, and between different types of arrays, Agilent versus Illumina. This approach has allowed us to confirm the consistency of our obtained results for a large number of genes that delineate the phenotypical differences of embryonic NSCs, and SN cells.
اللغةen
الناشرHamad bin Khalifa University Press (HBKU Press)
الموضوعGene Expression
Embryonic Human Neural Stem Cells
Dopaminergic Neurons
Adult Human Substantia Nigra
العنوانGene Expression Profiling of Embryonic Human Neural Stem Cells and Dopaminergic Neurons from Adult Human Substantia Nigra
النوعConference Paper
رقم العدد1
رقم المجلد2012
dc.accessType Abstract Only


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