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AuthorAhmed R.
AuthorAfreen A.
AuthorTariq M.
AuthorZahid A.A.
AuthorMasoud M.S.
AuthorAhmed M.
AuthorAli I.
AuthorAkram Z.
AuthorHasan, Anwarul
Available date2022-05-21T10:18:27Z
Publication Date2021
Publication NameBiomedical Materials (Bristol)
ResourceScopus
Identifierhttp://dx.doi.org/10.1088/1748-605X/abc28b
URIhttp://hdl.handle.net/10576/31272
AbstractImpaired diabetic wounds are one of the major pathophysiological complications caused by persistent microbial infections, prolonged inflammation, and insufficient angiogenic responses. Here, we report the development of nitric-oxide (NO) -releasing S-nitroso-N-acetyl-penicillamine (SNAP) -loaded chitosan/polyvinyl–alcohol hydrogel and its efficacy in enhancing the wound-healing potential of bone marrow mesenchymal stem cells in diabetic wounds. NO-releasing hydrogels significantly increased the cell viability and cell proliferation of hydrogen-peroxide (H2O2) -pretreated bone marrow stem cells (BMSCs), demonstrating their cytoprotective activity, which was further confirmed by gene expression of many times as much B-cell lymphoma 2 (Bcl-2), stromal cell-derived factor-1alpha (SDF-1α), proliferating cell nuclear antigen (PCNA) and vascular endothelial growth factor (VEGF). Furthermore, the SNAP-loaded hydrogel showed continuous cell-proliferating activity for six days, due to the slow release of NO from the hydrogel. Wound-healing studies of rabbits with induced diabetes showed that the application of SNAP-preconditioned BMSCs and NO-releasing hydrogels significantly sped up the healing process, compared to the control group. The wound-healing potential of BMSCs plus NO-releasing hydrogel was further validated by improved collagen deposition and epithelial layer formation, as confirmed by histopathological examination, as well as upregulation of VEGF and SDF-1α biomarkers, as evidenced by gene-expression analysis. These results demonstrated that the application of BMSCs with NO-releasing hydrogel can promote faster regeneration of damaged tissues. Therefore, BMSCs plus NO-releasing hydrogels can be very useful for the treatment of diabetic wounds.
Languageen
PublisherIOP Publishing Ltd
SubjectBone marrow stem cells
Diabetic wound healing
Hydrogels
Nitric oxide
SNAP
TitleBone marrow mesenchymal stem cells preconditioned with nitric-oxide-releasing chitosan/PVA hydrogel accelerate diabetic wound healing in rabbits
TypeArticle
Pagination350-358
Issue Number3
Volume Number16
dc.accessType Abstract Only


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