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المؤلفMehrabi M.
المؤلفGhasemi M.F.
المؤلفRasti B.
المؤلفFalahati M.
المؤلفMirzaie A.
المؤلفHasan, Anwarul
تاريخ الإتاحة2022-05-21T10:18:28Z
تاريخ النشر2021
اسم المنشورJournal of Biomolecular Structure and Dynamics
المصدرScopus
المعرّفhttp://dx.doi.org/10.1080/07391102.2020.1751296
معرّف المصادر الموحدhttp://hdl.handle.net/10576/31286
الملخصNanoporous iron oxide (Fe3O4) nanoparticles (NIONPs) have been widely used as promising agents in biomedical applications. Herein, the NIONPs were synthesized by one-step hydrothermal method and well-characterized by FESEM and TEM investigations. Afterwards, their interaction with human serum albumin (HSA) was studied using a wide range of biophysical approaches, including intrinsic and extrinsic fluorescence, far and near UV-CD, and UV-Vis spectroscopic methods as well as molecular docking investigation. Furthermore, the antibacterial effect of NIONPs was examined on the standard strains of the following pathogenic bacteria, Staphylococcus aureus (ATCC 25923), Klebsiella penumoniae (ATCC 33883), Enterococcus faecalis (ATCC 29212) and Pseudomonas aeruginosa (ATCC 27853). The results showed the feasible fabrication of spherical-shaped NIONPs with an average diameter of around 100nm. Intrinsic fluorescence spectroscopy data depicted that NIONPs formed a complex with HSA by a KSV value of 0.092 (�g/ml)?1. Extrinsic fluorescence, near UV-CD and UV-vis spectroscopic methods revealed that NIONPs induced some changes on the quaternary structure of HSA, whereas Tm measurement and far UV-CD spectroscopy showed some slight changes on the secondary structure of HSA even in the presence of high concentration of NIONPs. Molecular docking study disclosed that Fe3O4 nanoclusters with varying morphologies and dimensions could interact with different residues on the surface of HSA molecules. In addition, antibacterial assays exhibited a significant inhibition on both Gram-positive and Gram-negative pathogenic bacteria. In conclusion, these NPs can be used as promising antibacterial agents. Communicated by Ramaswamy H. Sarma.
اللغةen
الناشرTaylor and Francis Ltd.
الموضوعantibacterial
docking
nanoparticle
Nanoporous iron oxide
spectroscopy
العنوانNanoporous iron oxide nanoparticle: hydrothermal fabrication, human serum albumin interaction and potential antibacterial effects
النوعArticle
رقم العدد7
رقم المجلد39
dc.accessType Abstract Only


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