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المؤلفAugustine R.
المؤلفur Rehman S.R.
المؤلفK. S. J.
المؤلفHasan, Anwarul
تاريخ الإتاحة2022-05-21T10:18:29Z
تاريخ النشر2020
اسم المنشورRSC Advances
المصدرScopus
المعرّفhttp://dx.doi.org/10.1039/d0ra04997b
معرّف المصادر الموحدhttp://hdl.handle.net/10576/31296
الملخصChronic wounds are one of the key concerns for people with diabetes, frequently leading to infections and non-healing ulcers, and finally resulting in the amputation of limbs/organs. Stromal cell-derived factor 1 (SDF1) is a major chemokine that plays a significant role in tissue repair, vascularization, and wound healing. However, the long-term sustained delivery of SDF1 in a chronic wound environment is a great challenge. In order to facilitate the sustained release of SDF1 in diabetic wounds, it could be incorporated into wound-healing patches. Herein, we report the fabrication of a hydrophilic/hydrophobic bicomponent fiber-based membrane, where SDF1 was encapsulated inside hydrophilic fibers, and its applicability in wound healing. A co-electrospinning technique was employed for the fabrication of polymeric membranes where PVA and PCL form the hydrophilic and hydrophobic components, respectively. Morphological analysis of the developed membranes was conductedviascanning electron microscopy (SEM). The mechanical strength of the membranes was investigatedviauniaxial tensile testing. The water uptake capacity of the membranes was also determined to understand the hydrophilicity and exudate uptake capacity of the membranes. To understand the proliferation, viability, and migration of skin-specific cells in the presence of SDF1-loaded membranes,in vitrocell culture experiments were carried out using fibroblasts, keratinocytes, and endothelial cells. The results showed the excellent porous morphology of the developed membranes with distinguishable differences in fiber diameters for the PVA and PCL fibers. The developed membranes possessed enough mechanical strength for use as wound-healing membranes. The co-electrospun membranes showed good exudate uptake capacity. The controlled and extended delivery of SDF1 from the developed membranes was observed over a prolonged period. The SDF1-loaded membranes showed enhanced cell proliferation, cell viability, and cell migration. These biocompatible and biodegradable SDF1-loaded bicomponent membranes with excellent exudate uptake capacity, and cell proliferation and cell migration properties can be exploited as a novel wound-dressing membrane aimed at chronic diabetic wounds.
اللغةen
الناشرRoyal Society of Chemistry
الموضوعBiocompatibility
Cell culture
Cell proliferation
Endothelial cells
Fibers
Hydrophilicity
Tensile testing
Tissue regeneration
Bi-component fibers
Co-electrospinning
Electron microscopy (SEM)
Extended deliveries
Hydrophilic and hydrophobic
Hydrophilic fibers
Hydrophilic/hydrophobic
Morphological analysis
Membranes
العنوانStromal cell-derived factor loaded co-electrospun hydrophilic/hydrophobic bicomponent membranes for wound protection and healing
النوعArticle
رقم العدد1
رقم المجلد11
dc.accessType Open Access


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