Show simple item record

AuthorSharifi M.
AuthorSohrabi M.J.
AuthorHosseinali S.H.
AuthorHasan, Anwarul
AuthorKani P.H.
AuthorTalaei A.J.
AuthorKarim A.Y.
AuthorNanakali N.M.Q.
AuthorSalihi A.
AuthorAziz F.M.
AuthorYan B.
AuthorKhan R.H.
AuthorSaboury A.A.
AuthorFalahati M.
Available date2022-05-21T10:18:31Z
Publication Date2020
Publication NameInternational Journal of Biological Macromolecules
ResourceScopus
Identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2019.12.064
URIhttp://hdl.handle.net/10576/31314
AbstractNanoparticles (NPs) have been widely used for immobilization of wide ranges of enzymes. However, the stabilization of enzymes on NPs is a major challenge, crucial for regulating enzymatic activity and their medical applications. To overcome these challenges, it is necessary to explore how enzymes attach to nanomaterials and their properties are affected by such interactions. In this review we present an overview on the different strategies of the enzyme immobilization into the NPs and their corresponding stability against temperature and pH. The effects of surface charge, particle size, morphology, and aggregation of NPs on the stability of immobilized enzymes were summarized. The activity of immobilized enzyme into the NPs was reviewed to disclose more detail regarding the interaction of biomolecules with NPs. The combination of enzyme immobilization with prodrugs was also reviewed as a promising approach for biomedical application of enzyme in cancer therapy. Finally, the current challenges and future applications of NPs in enzyme immobilization and the utilization of immobilized enzyme toward prodrug activation in cytoplasm of cancer cells were presented. In conclusion, this review may pave the way for providing a perspective on development to the industrial and clinical translation of immobilized enzymes.
Languageen
PublisherElsevier B.V.
SubjectCancer treatment
Enzyme prodrug therapy
Enzyme stability
Immobilization
Nanomaterials
TitleEnzyme immobilization onto the nanomaterials: Application in enzyme stability and prodrug-activated cancer therapy
TypeArticle
Volume Number143
dc.accessType Abstract Only


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record