Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats
Author | Shakya, P. |
Author | Sharma, A.K. |
Author | Kumar, N. |
Author | Vellachi, R. |
Author | Mathew, D.D. |
Author | Dubey, P. |
Author | Singh, K. |
Author | Shrivastava, S. |
Author | Shrivastava, S. |
Author | Maiti S.K. |
Author | Hasan, Anwarul |
Author | Singh K.P. |
Available date | 2022-05-21T10:18:34Z |
Publication Date | 2016 |
Publication Name | Scientifica |
Resource | Scopus |
Identifier | http://dx.doi.org/10.1155/2016/2638371 |
Abstract | An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 ? 20 mm2) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P < 0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats. |
Language | en |
Publisher | Hindawi Limited |
Subject | Bubaline Cholecyst Derived Extracellular Matrix Reconstruction Rats |
Type | Article |
Volume Number | 2016 |
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Mechanical & Industrial Engineering [1342 items ]