Differences in platelet-rich plasma composition influence bone healing
Author | Al-Hamed F.S. |
Author | Abu-Nada L. |
Author | Rodan R. |
Author | Sarrigiannidis S. |
Author | Ramirez-Garcialuna J.L. |
Author | Moussa H. |
Author | Elkashty O. |
Author | Gao Q. |
Author | Basiri T. |
Author | Baca L. |
Author | Torres J. |
Author | Rancan L. |
Author | Tran S.D. |
Author | Lordkipanidz� M. |
Author | Kaartinen M. |
Author | Badran Z. |
Author | Tamimi F. |
Available date | 2022-05-31T19:01:18Z |
Publication Date | 2021 |
Publication Name | Journal of Clinical Periodontology |
Resource | Scopus |
Identifier | http://dx.doi.org/10.1111/jcpe.13546 |
Abstract | Aim: Platelet-rich plasma (PRP) is an autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP (L-PRP) composition on bone defect healing. Materials and Methods: L-PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP, double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro-CT and histology. Results: Double-spin L-PRP contained higher concentrations of platelets than single-spin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08 � 0.2 mm3) compared to single-spin L-PRP (1.42 � 0.27 mm3) or filtered L-PRP (1.38 � 0.28 mm3). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double-spin L-PRP than in those treated with single-spin or filtered L-PRP. Conclusion: The preparation method of L-PRP affects their composition and potential to regenerate bone |
Language | en |
Publisher | John Wiley and Sons Inc |
Subject | acid citrate dextrose acid phosphatase tartrate resistant isoenzyme alcohol calcium calcium chloride carbon dioxide carprofen collagen eg1160 eosin hematoxylin iron magnesium paraffin potassium selenium serotonin sodium tolonium chloride water analgesia animal experiment animal model animal tissue Article blood cell count blood sampling bone defect bone mineral bone regeneration bone volume fraction cell infiltration centrifugation citric acid cycle cortical thickness (bone) dehydration dialysis enzyme linked immunosorbent assay erythrocyte female filtration fracture healing histology histopathology inductively coupled plasma mass spectrometry leukocyte liquid chromatography-mass spectrometry lymphocyte macrophage mass fragmentography mast cell metabolite micro-computed tomography nonhuman orthopedic surgery osteoclast oxidative stress paraffin embedding pentose phosphate cycle platelet count puncture rat serotonin blood level thrombocyte rich plasma tibia trabecular number trabecular separation trabecular thickness animal connective tissue Animals Bone Regeneration Collagen Connective Tissue Platelet-Rich Plasma Rats Tibia |
Type | Article |
Pagination | 1613-1623 |
Issue Number | 12 |
Volume Number | 48 |
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