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Improvement of Bacillus thuringiensis bioinsecticide production by sporeless and sporulating strains using response surface methodology
(
Elsevier B.V.
, 2011 , Article)
Statistical experimental designs, involving a Plackett-Burman design followed by a rotatable central composite design were used to optimize the culture medium constituents for Bacillus thuringiensis bioinsecticide production. ...
Involvement of oxidative stress and growth at high cell density in the viable but nonculturable state of Photorhabdus temperata ssp. temperata strain K122
(
Elsevier Ltd
, 2010 , Article)
Photorhabdus temperata ssp. temperata strain K122 represents a promising source of bioinsecticide. When cultured in an optimized medium, P. temperata exhibited restricted survival in terms of colony-forming ability on solid ...
Effects of the P20 protein from Bacillus thuringiensis israelensis on insecticidal crystal protein Cry4Ba
(
Elsevier B.V.
, 2015 , Article)
The accessory protein P20 from Bacillus thuringiensis israelensis has been defined as an important molecular chaperone for forming crystal Cyt1Aa, and enhancing Cry11Aa and Cry4Aa expression. To investigate its putative ...
Construction of a Bacillus thuringiensis genetically-engineered strain harbouring the secreted Cry1Ia delta-endotoxin in its crystal
(
Springer Netherlands
, 2011 , Article)
Unlike other Bacillus thuringiensis Cry proteins, Cry1 Ia does not form a crystal since it is a secreted delta-endotoxin. We have engineered a Cry1 Iac chimeric protein by substituting the C-terminal part of Cry1 Ia by the ...
Chemical composition, cytotoxicity effect and antimicrobial activity of Ceratonia siliqua essential oil with preservative effects against Listeria inoculated in minced beef meat
(
Elsevier B.V.
, 2011 , Article)
The present study describes the phytochemical profile and the protective effects of Ceratonia siliqua pods essential oil (CsEO), a food and medicinal plant widely distributed in Tunisia. Twenty five different components ...
Integration of a Recombinant Chitinase into Bacillus thuringiensis Parasporal Insecticidal Crystal
(
Springer-Verlag
, 2011 , Article)
Chitinases have been successfully used in combination with Bacillus thuringiensis delta-endotoxins forming crystals in order to enhance their insecticidal activities. In this context, we opted for promoting the chitinase ...
Study of the Bacillus thuringiensis Vip3Aa16 histopathological effects and determination of its putative binding proteins in the midgut of Spodoptera littoralis
(
Elsevier Inc.
, 2011 , Article)
The bacterium Bacillus thuringiensis produces, at the vegetative stage of its growth, Vip3A proteins with activity against a broad spectrum of lepidopteran insects. The Egyptian cotton leaf worm (Spodoptera littoralis) is ...
Overproduction of Delta-Endotoxins by Sporeless Bacillusthuringiensis Mutants Obtained by Nitrous Acid Mutagenesis
(
Springer-Verlag
, 2011 , Article)
Asporogenic and oligosporogenic Bacillus thuringiensis mutants having the ability to overproduce insecticidal crystal protein were generated by using nitrous acid (50 mg/ml), as chemical mutagenic agent. Insecticidal crystal ...
Relationship between plasmid loss and gene expression in Bacillus thuringiensis
(
Springer-Verlag
, 2011 , Article)
The large genome of Bacillus thuringiensis was shortened by provoking plasmid loss, and the effect of such loss on the expression of both chromosomal and plasmid-harboured genes was studied. It was evidenced that the genomic ...
Antifungal activities of an endophytic Pseudomonas fluorescens strain Pf1TZ harbouring genes from pyoluteorin and phenazine clusters
(
Springer Netherlands
, 2010 , Article)
Pseudomonas fluorescens Pf1TZ was inhibitory in vitro to a number of phytopathogenic fungi and could protect vine plantlets against Botrytis cinerea. Total protection was reached after 3 weeks of bacterial inoculation. The ...