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Integration of a Recombinant Chitinase into Bacillus thuringiensis Parasporal Insecticidal Crystal

Driss, Fatma; Rouis, Souad; Azzouz, Hichem; Tounsi, Slim; Zouari, Nabil; ... more authors ... less authors ( Springer-Verlag , 2011 , Article)

Chitinases have been successfully used in combination with Bacillus thuringiensis delta-endotoxins forming crystals in order to enhance their insecticidal activities. In this context, we opted for promoting the chitinase ...

Study of the Bacillus thuringiensis Vip3Aa16 histopathological effects and determination of its putative binding proteins in the midgut of Spodoptera littoralis

Abdelkefi-Mesrati, Lobna; Boukedi, Hanen; Dammak-Karray, Mariam; Sellami-Boudawara, Tahya; Jaoua, Samir; ... more authors ... less authors ( Elsevier Inc. , 2011 , Article)

The bacterium Bacillus thuringiensis produces, at the vegetative stage of its growth, Vip3A proteins with activity against a broad spectrum of lepidopteran insects. The Egyptian cotton leaf worm (Spodoptera littoralis) is ...

Construction of a Bacillus thuringiensis genetically-engineered strain harbouring the secreted Cry1Ia delta-endotoxin in its crystal

Dammak, Mariam; Jaoua, Samir; Tounsi, Slim ( Springer Netherlands , 2011 , Article)

Unlike other Bacillus thuringiensis Cry proteins, Cry1 Ia does not form a crystal since it is a secreted delta-endotoxin. We have engineered a Cry1 Iac chimeric protein by substituting the C-terminal part of Cry1 Ia by the ...

Susceptibility of Agrotis segetum (noctuidae) to Bacillus thuringiensis and analysis of midgut proteinases

Ben Hamadou-Charfi, Dorra; Sauer, Annette Juliane; Abdelkefi-Mesrati, Lobna; Tounsi, Slim; Jaoua, Samir; ... more authors ... less authors ( Taylor & Francis , 2015 , Article)

Seventy-eight Bacillus thuringiensis isolates were selected for a screening against the Lepidoptera species Agrotis segetum to search the higher insecticidal activity. In a preliminary bioassay, the spore-crystal mixture ...

Evidence of two mechanisms involved in Bacillus thuringiensis israelensis decreased toxicity against mosquito larvae: Genome dynamic and toxins stability

Elleuch, Jihen; Zribi Zghal, Raida; Lacoix, Marie Noel; Chandre, Fabrice; Tounsi, Slim; ... more authors ... less authors ( Elsevier GmbH , 2015 , Article)

Biopesticides based on Bacillus thuringiensis israelensis are the most used and most successful around the world. This bacterium is characterized by a dynamic genome able to win or lose genetic materials which leads to a ...

Cry4Ba and Cyt1Aa proteins from Bacillus thuringiensis israelensis: Interactions and Toxicity mechanism against Aedes aegypti

Elleuch, Jihen; Jaoua, Samir; Darriet, Frederic; Chandre, Fabrice; Tounsi, Slim; ... more authors ... less authors ( Elsevier Ltd , 2015 , Article)

Individual crystal proteins from Bacillus thuringiensis israelensis exhibit variable levels of insecticidal activities against mosquito larvae. In all cases, they are much less active compared to the whole crystal proteins ...

Relationship between plasmid loss and gene expression in Bacillus thuringiensis

Driss, Fatma; Tounsi, Slim; Jaoua, Samir ( Springer-Verlag , 2011 , Article)

The large genome of Bacillus thuringiensis was shortened by provoking plasmid loss, and the effect of such loss on the expression of both chromosomal and plasmid-harboured genes was studied. It was evidenced that the genomic ...

Chemical composition and evaluation of antioxidant and antimicrobial activities of Tunisian Thymelaea hirsuta with special reference to its mode of action

Trigui, Mohamed; Ben Hsouna, Anis; Tounsi, Slim; Jaoua, Samir ( Elsevier B.V. , 2013 , Article)

In the present study, we evaluated in vitro antimicrobial and antioxidant properties of Thymelaea hirsuta extracts as well as its phytochemical composition. Total phenolic and flavonoid contents were highest in acetone and ...

Potential of Photorhabdus temperata K122 bioinsecticide in protecting wheat flour against Ephestia kuehniella

Jallouli, Wafa; Abdelkefi-Mesrati, Lobna; Tounsi, Slim; Jaoua, Samir; Zouari, Nabil ( Elsevier Ltd , 2013 , Article)

The present study reports investigations on the insecticidal activity of the entomopathogenic bacterium Photorhabdus temperata K122 against the Mediterranean flour moth Ephestia kuehniella. Cultured in the optimized medium, ...

Agrotis segetum midgut putative receptor of Bacillus thuringiensis vegetative insecticidal protein Vip3Aa16 differs from that of Cry1Ac toxin

Ben Hamadou-Charfi, Dorra; Boukedi, Hanen; Abdelkefi-Mesrati, Lobna; Tounsi, Slim; Jaoua, Samir ( Elsevier Inc. , 2013 , Article)

Considering the fact that Agrotis segetum is one of the most pathogenic insects to vegetables and cereals in the world, particularly in Africa, the mode of action of Vip3Aa16 of Bacillus thuringiensis BUPM95 and Cry1Ac of ...