عرض بسيط للتسجيلة

المؤلفAl-Thani, Asma A.
المؤلفHamdi, Wedad S.
المؤلفAl-Ansari, Naser A.
المؤلفDoiphode, Sanjay H.
تاريخ الإتاحة2015-10-28T10:14:23Z
تاريخ النشر2014
اسم المنشورBMC infectious diseases
الاقتباسAl-Thani, A.,Hamdi, W., Al-Ansari, N., Doiphode, S.(2014).Polymerase chain reaction ribotyping of Clostridium difficile isolates in Qatar: a hospital-based study.BMC infectious diseases, 14(1):502
الرقم المعياري الدولي للكتاب1471-2334
معرّف المصادر الموحدhttps://dx.doi.org/10.1186/1471-2334-14-502
معرّف المصادر الموحدhttp://hdl.handle.net/10576/3600
الملخصBackground Clostridium difficile infection (CDI) is not generally reported to public health authorities in the Middle East and its true prevalence remains largely unknown. The aims of this study were to determine the prevalence of CDI and its associated ribotypes among C. difficile isolates in Qatar. Influence of age and correlation with other risk factors e.g. proton pump inhibitor use, antibiotic use, existence of chronic conditions, etc was also investigated for CDI positive patients. Methods A total of 1,532 patients with suspected CDI were recruited from two hospitals between 2011 and 2012. C. difficile was identified using glutamate dehydrogenase (GDH) lateral flow assay and toxins A and B Enzyme Immunoassay (EIA). The C. difficile positive samples were then cultured for PCR-ribotyping. Results 122 of the 1,532 (7.9%) samples from individual patients were identified as C.difficile positive; and 79 of these were viably cultured (~65%). From these, 36 different PCR ribotypes were isolated, of which strains 258 (6 [7.6%]), 01/014/046 (5 [6.3%]), and 011/053/056/107 (4 [5%]) were the most prevalent. The prevalence of PCR-ribotype 027 was 1.3% (n = 1). An age of ≥65 years and treatment with proton pump inhibitors correlated with higher frequency of CDI. Treatment with third generation cephalosporins (50 [41%]) and piperacillin/tazobactam antibiotics (55 [45.1%]) was most frequently associated with CDI. Conclusion The most common C. difficile ribotype identified in Qatar was 258, which is different from those found in North America, Europe and Asia. The prevalence of CDI was higher in Qatar than Europe; though comparable to other Middle Eastern countries. These findings underscore the importance of local surveillance to detect and control C. difficile infection.
اللغةen
الناشرBioMed Central Ltd.
الموضوعClostridium difficile
الموضوعRibotype
الموضوعQatar
العنوانPolymerase chain reaction ribotyping of Clostridium difficile isolates in Qatar: a hospital-based study
النوعArticle
رقم المجلد14


الملفات في هذه التسجيلة

Icon

هذه التسجيلة تظهر في المجموعات التالية

عرض بسيط للتسجيلة