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AuthorBatista, F. M.
AuthorLopez-Sanmartin, M.
AuthorBoudry, P.
AuthorNavas, J. I.
AuthorRuano, F.
AuthorRenault, T.
AuthorFonseca, V. G.
AuthorLeitao, A.
Available date2015-11-08T10:27:57Z
Publication Date2014
Publication NameAquaculture Research
CitationBatista, F. M., Lopez-Sanmartin, M., Boudry, P., Navas, J. I., Ruano, F., Renault, T., Fonseca, V. G. and Leitao, A. (2014), Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids. Aquaculture Research.
ISSN1365-2109
URIhttp://dx.doi.org/10.1111/are.12613
URIhttp://hdl.handle.net/10576/3785
AbstractCytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid respectively. Sequencing analysis of a viral fragment amplified with the C2/C6 primer pair revealed a high similarity with the OsHV-1 reference type. Moreover, in situ hybridization confirmed the presence of OsHV-1 in gill tissue. Oysters where OsHV-1 was detected had a significantly higher mean percentage of aneuploid cells (25%) than the ones where the virus was not detected (18%). However, the overall low percentage of positive samples contrasted with the high mean percentage of aneuploidy observed, with 50% of the oysters analysed showing a percentage of aneuploid cells between 20% and 30%. We hypothesize that somatic aneuploidy may adversely affect oysters making them more prone to OsHV-1 infection, but the virus is unlikely to be the cause of somatic aneuploidy.
SponsorFCT. Grant Number: SFRH/BD/8972/2002
SponsorMarie Curie Training fellowship. Grant Numbers: PLUDAMOR, QLK5-CT-2000-60036
Languageen
PublisherJohn Wiley & Sons Ltd
Subjectaneuploidy
Subjectostreid herpesvirus 1
Subjectoyster
SubjectCrassostrea
TitleInsights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids
TypeArticle


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