عرض بسيط للتسجيلة

المؤلفAbdulwahab, Atqah
المؤلفTaj-Aldeen, Saad J.
المؤلفDoiphode, S
المؤلفAbdulla, Shaikha H.
المؤلفMuhammed, Ramees
المؤلفAhmed, Irshad
المؤلفAbdeen, Yasmine
المؤلفSadek, Omnia
المؤلفAbu-Madi, Marawan
تاريخ الإتاحة2015-11-09T10:29:33Z
تاريخ النشر2014-11-20
اسم المنشورInfection and Drug Resistance
الاقتباسAbdulWahab, A., Taj-Aldeen, S. J., Ibrahim, E., Abdulla, S. H., Muhammed, R., Ahmed, I., Abdeen, Y., Sadek, O., Abu-Madi, M. (2014).Genetic relatedness and host specificity of Pseudomonas aeruginosa isolates from cystic fibrosis and non-cystic fibrosis patients. Infection and Drug Resistance, 7 : 309 - 316
الرقم المعياري الدولي للكتاب1178-6973
معرّف المصادر الموحدhttp://dx.doi.org/10.2147/IDR.S72112
معرّف المصادر الموحدhttp://hdl.handle.net/10576/3805
الملخصBackground: Pseudomonas aeruginosa is one of the primary pathogens isolated more frequently in cystic fibrosis (CF) and it exhibits innate resistance to a wide range of antibiotics. Purpose: We sought to determine whether the highly prevalent genotypes of P. aeruginosa are specifically linked to CF patients and have any related multidrug antibiotic resistance. Isolates from hospitalized non-CF patients and from environmental sources were also genotypically analyzed. Methods: Collections of P. aeruginosa from lower respiratory secretions (n=45) were genotyped using pulsed-field gel electrophoresis (PFGE). Phenotypic screening for antibiotic susceptibility was performed for the common antimicrobial agents by E-test and automated Phoenix method. Results: P. aeruginosa isolates from CF (n=32), hospitalized non-CF patients (n=13), and environment sources (n=5) were analyzed. The population structure of P. aeruginosa is highly diverse and population-specific. All PFGE results of P. aeruginosa isolates fall among four major clusters. Cluster 1 contained 16 P. aeruginosa isolates from CF patients and two from environmental sources; cluster 2 contained 11 P. aeruginosa isolates from CF and one each from non-CF and environmental sources; cluster 3 contained 12 P. aeruginosa isolates from hospitalized non-CF patients and two P. aeruginosa isolates from one CF patient and one environmental source; and cluster 4 consisted of three isolates from CF patients and one from the environment. The majority of multidrug-resistant P. aeruginosa isolates were in clusters 3 and 4. P. aeruginosa isolates from CF patients were resistant to ciprofloxacin (34.4%) followed by resistance to amikacin and gentamicin (each 28%), whereas the majority of isolates from non-CF patients were resistant to meropenem (69%) and were grouped in cluster 3. Conclusion: PFGE of P. aeruginosa isolates from CF patients shows a high degree of similarity, suggesting specific adaptation of these clones to CF-affected lungs. The hospitalized non-CF cluster has a different clonal origin, indicating specific clustering in a specific location, suggesting hospital-acquired P. aeruginosa infections.
راعي المشروعThe Qatar National Research Fund (UREP 14-026-3-010).
اللغةen
الناشرDove Medical Press Limited
الموضوعcystic fibrosis
drug susceptibility testing
Pseudomonas aeruginosa
pulsed-field gel electrophoresis
العنوانGenetic relatedness and host specificity of Pseudomonas aeruginosa isolates from cystic fibrosis and non-cystic fibrosis patients
النوعArticle
الصفحات309 - 316
رقم المجلد7
dc.accessType Open Access


الملفات في هذه التسجيلة

Thumbnail

هذه التسجيلة تظهر في المجموعات التالية

عرض بسيط للتسجيلة