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المؤلفJallouli, Wafa
المؤلفZouari, Nabil
المؤلفJaoua, Samir
تاريخ الإتاحة2015-12-16T10:14:58Z
تاريخ النشر2010-05
اسم المنشورProcess Biochemistryen_US
المعرّفhttp://dx.doi.org/10.1016/j.procbio.2010.01.007
الاقتباسInvolvement of oxidative stress and growth at high cell density in the viable but nonculturable state of Photorhabdus temperata ssp. temperata strain K122 Jallouli, Wafa; Zouari, Nabil; Jaoua, Samir, (2010) Process Biochemistry, vol. 45(5), p. 706-713
الرقم المعياري الدولي للكتاب1359-5113
الرقم المعياري الدولي للكتاب1873-3298 (online)
معرّف المصادر الموحدhttp://hdl.handle.net/10576/3963
الملخصPhotorhabdus temperata ssp. temperata strain K122 represents a promising source of bioinsecticide. When cultured in an optimized medium, P. temperata exhibited restricted survival in terms of colony-forming ability on solid medium, which remained lower than the total cell counts. Membrane integrity assessment by flow cytometry showed that almost 100% of P. temperata cells were viable indicating that this bacterium enters in the viable but nonculturable state (VBNC). According to the double staining results, hydrogen peroxide was demonstrated to be responsible of P. temperata VBNC state. Addition of catalase or sodium pyruvate upon the inoculation of P. temperata on agar plates promoted the recovery of nonculturable cells up to 24 h incubation. Further, growth at high cell density enhanced the VBNC state of this bacterium. This should evidenced extracellular signals accumulation involved in quorum sensing mechanism. Elucidation of this state is interesting for both toxicity study and production of P. temperata useful as bioinsecticide.
راعي المشروعTunisian Ministère de l’Enseignement Supérieur, de la Recherche Scientifique et de la Technologie (MESRS).
اللغةen
الناشرElsevier Ltd.
الموضوعPhotorhabdus temperata
الموضوعNonculturable cells
الموضوعHigh cell density
الموضوعCatalase
الموضوعSodium pyruvate
الموضوعH2O2
الموضوعFlow cytometry
العنوانInvolvement of oxidative stress and growth at high cell density in the viable but nonculturable state of Photorhabdus temperata ssp. temperata strain K122
النوعArticle
الصفحات706-713
رقم العدد5
رقم المجلد45


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