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المؤلفSaadaoui, Imen
المؤلفMiled, Nabil
المؤلفJaoua, Samir
تاريخ الإتاحة2015-12-22T09:54:57Z
تاريخ النشر2010-05
اسم المنشورMolecular Biotechnologyen_US
الاقتباسSaadaoui, I., Miled, N., & Jaoua, S., (2010), "Evidence of the Involvement of E358 , A498 and C571 of a New Cry1Ac d -endotoxin of Bacillus thuringiensis in its High Insecticidal Activity Against Ephestia kuehniella", Molecular Biotechnology 45(1), 65–70
الرقم المعياري الدولي للكتاب1073-6085
الرقم المعياري الدولي للكتاب1559-0305 (online)
معرّف المصادر الموحدhttp://dx.doi.org/10.1007/s12033-010-9243-z
معرّف المصادر الموحدhttp://hdl.handle.net/10576/3973
الملخصA new cry1Ac-type gene was cloned from Bacillus thuringiensis strain BLB1, sequenced and expressed. The deduced amino acid sequence of the polypeptide has a predicted molecular mass of 132.186 kDa. The amino acid sequence alignment of BLB1 Cry1Ac with those of the published ones showed that this is a new delta-endotoxin. When compared with Cry1Ac of Bacillus thuringiensis strain HD1, it was found that BLB1 Cry1Ac harbours three mutations: V358E localized in domain II and V498A and Y571C localized in domain III. When the BLB1 Cry1Ac toxin was expressed in an acrystalliferous strain of B. thuringiensis (HD1CryB), bipyramidal crystals were produced. The spore-crystal mixture of this recombinant strain was at least two-fold more active against larvae of the lepidopteran Ephestia kuehniella than that of the recombinant strain expressing Cry1Ac of HD1. The study of the structural effect of these mutations suggested that they may stabilize key regions involved in the binding of the domains II and III to insect receptors.
راعي المشروعTunisian Ministry of Higher Education, Scientific Research and Technology, "Departamento de Genetica, Fac. de Ciencias Biologicas, Universitat de Valencia, Spain" from the Federation of the Societies of Biochemistry and Molecular Biology (FEBS).
اللغةen
الناشرHumana Press Inc
الموضوعcry1Ac gene
الموضوعDelta-endotoxin structure
الموضوعBacillus thuringiensis
الموضوعEphestia kuehniella
العنوانEvidence of the Involvement of E358, A498 and C571 of a New Cry1Ac δ-endotoxin of Bacillus thuringiensis in its High Insecticidal Activity Against Ephestia kuehniella
النوعArticle
الصفحات65-70
رقم العدد1
رقم المجلد45


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