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AuthorMarei, Hany E.
AuthorFarag, Amany
AuthorAl-Thani, Asma
AuthorAfifi, Nahla
AuthorAbd-Elmaksoud, Ahmed
AuthorLashen, Samah
AuthorRezk, Shaymaa
AuthorPallini, Roberto
AuthorCasalbore, Patrizia
AuthorCenciarelli, Carlo
Available date2015-12-27T10:55:49Z
Publication Date2015-01
Publication NameJournal of Cellular Physiologyen_US
CitationMarei, Hany E. S., et al, "Human Olfactory Bulb Neural Stem Cells expressing hNGF Restore Cognitive Deficit in Alzheimer's Disease Rat Model", Journal of Cellular Physiology, 2015, 230(1):116-130
ISSN1097-4652 (online)
AbstractIn this study, we aim to demonstrate the fate of allogenic adult human olfactory bulb neural stem/progenitor cells (OBNSC/NPCs) transplanted into the rat hippocampus treated with ibotenic acid (IBO), a neurotoxicant specific to hippocampal cholinergic neurons that are lost in Alzheimer’s disease. We assessed their possible ability to survive, integrate, proliferate, and differentiate into different neuronal and glial elements: we also evaluate their possible therapeutic potential, and the mechanism(s) relevant to neuroprotection following their engraftment into the CNS milieu. OBNSC/NPCs were isolated from adult human olfactory bulb patients, genetically engineered to express GFP and human nerve growth factor (hNGF) by lentivirus-mediated infection, and stereotaxically transplanted into the hippocampus of IBO-treated animals and controls. Stereological analysis of engrafted OBNSCs eight weeks post transplantation revealed a 1.89 fold increase with respect to the initial cell population, indicating a marked ability for survival and proliferation. In addition, 54.71 11.38%, 30.18 6.00%, and 15.09 5.38% of engrafted OBNSCs were identified by morphological criteria suggestive of mature neurons, oligodendrocytes and astrocytes respectively. Taken together, this work demonstrated that human OBNSCs expressing NGF ameliorate the cognitive deficiencies associated with IBO-induced lesions in AD model rats, and the improvement can probably be attributed primarily to neuronal and glial cell replacement as well as the trophic influence exerted by the secreted NGF.
SponsorSTDF, Egypt and ATENA Onlus (Italy)
SubjectHuman Olfactory Bulb
SubjectAlzheimer's Disease treatment
TitleHuman Olfactory Bulb Neural Stem Cells Expressing hNGF Restore Cognitive Deficit in Alzheimer's Disease Rat Model
Issue Number1
Volume Number230

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