عرض بسيط للتسجيلة

المؤلفGentile, Adriana-Mariel
المؤلفLhamyani, Said
المؤلفCoín-Aragüez, Leticia
المؤلفOliva-Olivera, Wilfredo
المؤلفZayed, Hatem
المؤلفVega-Rioja, Antonio
المؤلفMonteseirin, Javier
المؤلفRomero-Zerbo, Silvana-Yanina
المؤلفTinahones, Francisco-José
المؤلفBermúdez-Silva, Francisco-Javier
المؤلفEl Bekay, Rajaa
تاريخ الإتاحة2016-10-19T07:36:44Z
تاريخ النشر2016-06-15
اسم المنشورPLoS ONEen_US
المعرّفhttp://dx.doi.org/10.1371/journal.pone.0157002
الاقتباسGentile A-M, Lhamyani S, Coín-Aragüez L, Oliva-Olivera W, Zayed H, Vega-Rioja A, et al. (2016) RPL13A and EEF1A1 Are Suitable Reference Genes for qPCR during Adipocyte Differentiation of Vascular Stromal Cells from Patients with Different BMI and HOMA-IR. PLoS ONE 11(6): e0157002.
معرّف المصادر الموحدhttp://hdl.handle.net/10576/4904
الملخصReal-time or quantitative PCR (qPCR) is a useful technique that requires reliable reference genes for data normalization in gene expression analysis. Adipogenesis is among the biological processes suitable for this technique. The selection of adequate reference genes is essential for qPCR gene expression analysis of human Vascular Stromal Cells (hVSCs) during their differentiation into adipocytes. To the best of our knowledge, there are no studies validating reference genes for the analyses of visceral and subcutaneous adipose tissue hVSCs from subjects with different Body Mass Index (BMI) and Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) index. The present study was undertaken to analyze this question. We first analyzed the stability of expression of five potential reference genes: CYC, GAPDH, RPL13A, EEF1A1, and 18S ribosomal RNA, during in vitro adipogenic differentiation, in samples from these types of patients. The expression of RPL13A and EEF1A1 was not affected by differentiation, thus being these genes the most stable candidates, while CYC, GAPDH, and 18S were not suitable for this sort of analysis. This work highlights that RPL13A and EEF1A1 are good candidates as reference genes for qPCR analysis of hVSCs differentiation into adipocytes from subjects with different BMI and HOMA-IR.
راعي المشروعInstituto de Salud Carlos III (PI10/01947, PI13/02628) with Fondos FEDER and the Consejería de Economía e Innovación, Ciencia y Empleo, Junta de Andalucía (CTS-7895) with Fondos FEDER. R. El Bekay is under a contract Miguel Servet type II (CPII13/00041) from the Instituto de Salud Carlos III. F-JB-S is a recipient of a "Miguel Servet II" research contract (CPII13/00042) and also belongs to the regional "Nicolás Monardes" research program of the Consejería de Salud (C-0070-2012; Junta de Andalucía, Spain). This work was supported by the FIS-Thematic Networks and Co-Operative Research Centres RIRAAF (RD07-0064). JM is under the Programa de Intensificación de la Actividad Investigadora del Sistema Nacional de Salud. AV-R is under a contract Proyectos de I+D+i para jóvenes investigadores from the Ministerio de Economía y Competitividad (SAF2014-60649-JIN). S-YR-Z is recipient of a post-doctoral contract from Consejería de Salud de la Junta de Andalucía (RH-0070-2013).
اللغةen
الناشرPublic Library of Science (PLoS)
الموضوعInsulin resistance
الموضوعGene expression
الموضوعMorbid obesity
الموضوعAdipose tissue
الموضوعAdipocyte differentiation
العنوانRPL13A and EEF1A1 Are Suitable Reference Genes for qPCR during Adipocyte Differentiation of Vascular Stromal Cells from Patients with Different BMI and HOMA-IR
النوعArticle
رقم العدد6
رقم المجلد11
ESSN1932-6203


الملفات في هذه التسجيلة

Icon
Icon

هذه التسجيلة تظهر في المجموعات التالية

عرض بسيط للتسجيلة