The treatment of bacterial biofilms cultivated on knee arthroplasty implants using the bioelectric effect
Author | Tamimi, Iskandar |
Author | Gasca, María |
Author | Halbardier, Alexandra |
Author | Martin, Sergio |
Author | Martin Caballero, Gregorio |
Author | Lucena Serrano, Cristina |
Author | Martin, Elena |
Author | Tamimi, Faleh |
Author | González-Quevedo, David |
Author | García de Quevedo, David |
Author | Sobrino, Beatriz |
Author | Palop, Begoña |
Author | Guerado, Enrique |
Author | Pérez Lara, Almudena |
Author | Urdiales, Cristina |
Author | Gómez de Gabriel, Jesús Manuel |
Available date | 2024-11-03T07:19:18Z |
Publication Date | 2024-07-02 |
Publication Name | Frontiers in Bioengineering and Biotechnology |
Identifier | http://dx.doi.org/10.3389/fbioe.2024.1426388 |
Citation | Tamimi, I., Gasca, M., Halbardier, A., Martin, S., Martin Caballero, G., Lucena Serrano, C., ... & Gómez De Gabriel, J. M. The treatment of bacterial biofilms cultivated on knee arthroplasty implants using the bioelectric effect. Frontiers in Bioengineering and Biotechnology, 12, 1426388. |
ISSN | 2296-4185 |
Abstract | Introduction: The formation of bacterial biofilms on knee arthroplasty implants can have catastrophic consequences. The aim of this study was to analyze the effectiveness of the bioelectric effect in the elimination of bacterial biofilms on cultivated knee arthroplasty implants. Methods: A novel device was designed to deliver a bioelectric effect on the surface of knee arthroplasty implants. 4-femoral prosthetic implants were cultivated with a staphylococcus aureus inoculum for 15 days. The components were divided into four different groups: A (not treated), B (normal saline 20-minutes), C (bioelectric effect 10-minutes), D (bioelectric effect 20-minutes). The implants were sonicated, and the detached colonies were quantified as the number of colony-forming unit (CFUs). The implants were sterilised and the process was repeated in a standardized manner four more times, to obtain a total of five samples per group. Results: The number of the CFUs after a 10-minute exposure to the bioelectric effect was of 208.2 ± 240.4, compared with 6,041.6 ± 2010.7 CFUs in group A, representing a decrease of 96.5% ± 4.3 (p = 0.004). And a diminution of 91.8% ± 7.9 compared with 2,051.0 ± 1,364.0 CFUs in group B (p = 0.109). The number of bacterial colonies after a 20-minute exposure to the bioelectric effect was 70 ± 126.7 CFUs, representing a decrease of 98.9% ± 1.9 (p = 0.000) compared with group A. And a decrease of 97.8% ± 3.0 (p = 0.019) compared with group B. Conclusions: The bioelectric effect was effective in the elimination of bacterial biofilm from knee arthroplasty implants. This method could be used in the future as part of conventional surgical procedures. |
Sponsor | This study was funded by the Spanish Government PID2021-127221OB-I00 and the Research Institute\u2019s Own Plan Malaga Biomedical and Nanomedicine Platform (IBIMA. Plataform BIONAND 2022). |
Language | en |
Publisher | Frontiers Media S.A. |
Subject | bioelectric effect biofilm periprosthetic infection Staphylococcus aureus total knee replacement |
Type | Article |
Volume Number | 12 |
Files in this item
This item appears in the following Collection(s)
-
Dental Medicine Research [330 items ]