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AuthorTamimi, Iskandar
AuthorGasca, María
AuthorHalbardier, Alexandra
AuthorMartin, Sergio
AuthorMartin Caballero, Gregorio
AuthorLucena Serrano, Cristina
AuthorMartin, Elena
AuthorTamimi, Faleh
AuthorGonzález-Quevedo, David
AuthorGarcía de Quevedo, David
AuthorSobrino, Beatriz
AuthorPalop, Begoña
AuthorGuerado, Enrique
AuthorPérez Lara, Almudena
AuthorUrdiales, Cristina
AuthorGómez de Gabriel, Jesús Manuel
Available date2024-11-03T07:19:18Z
Publication Date2024-07-02
Publication NameFrontiers in Bioengineering and Biotechnology
Identifierhttp://dx.doi.org/10.3389/fbioe.2024.1426388
CitationTamimi, I., Gasca, M., Halbardier, A., Martin, S., Martin Caballero, G., Lucena Serrano, C., ... & Gómez De Gabriel, J. M. The treatment of bacterial biofilms cultivated on knee arthroplasty implants using the bioelectric effect. Frontiers in Bioengineering and Biotechnology, 12, 1426388.
ISSN2296-4185
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85198526152&origin=inward
URIhttp://hdl.handle.net/10576/60793
AbstractIntroduction: The formation of bacterial biofilms on knee arthroplasty implants can have catastrophic consequences. The aim of this study was to analyze the effectiveness of the bioelectric effect in the elimination of bacterial biofilms on cultivated knee arthroplasty implants. Methods: A novel device was designed to deliver a bioelectric effect on the surface of knee arthroplasty implants. 4-femoral prosthetic implants were cultivated with a staphylococcus aureus inoculum for 15 days. The components were divided into four different groups: A (not treated), B (normal saline 20-minutes), C (bioelectric effect 10-minutes), D (bioelectric effect 20-minutes). The implants were sonicated, and the detached colonies were quantified as the number of colony-forming unit (CFUs). The implants were sterilised and the process was repeated in a standardized manner four more times, to obtain a total of five samples per group. Results: The number of the CFUs after a 10-minute exposure to the bioelectric effect was of 208.2 ± 240.4, compared with 6,041.6 ± 2010.7 CFUs in group A, representing a decrease of 96.5% ± 4.3 (p = 0.004). And a diminution of 91.8% ± 7.9 compared with 2,051.0 ± 1,364.0 CFUs in group B (p = 0.109). The number of bacterial colonies after a 20-minute exposure to the bioelectric effect was 70 ± 126.7 CFUs, representing a decrease of 98.9% ± 1.9 (p = 0.000) compared with group A. And a decrease of 97.8% ± 3.0 (p = 0.019) compared with group B. Conclusions: The bioelectric effect was effective in the elimination of bacterial biofilm from knee arthroplasty implants. This method could be used in the future as part of conventional surgical procedures.
SponsorThis study was funded by the Spanish Government PID2021-127221OB-I00 and the Research Institute\u2019s Own Plan Malaga Biomedical and Nanomedicine Platform (IBIMA. Plataform BIONAND 2022).
Languageen
PublisherFrontiers Media S.A.
Subjectbioelectric effect
biofilm
periprosthetic infection
Staphylococcus aureus
total knee replacement
TitleThe treatment of bacterial biofilms cultivated on knee arthroplasty implants using the bioelectric effect
TypeArticle
Volume Number12
dc.accessType Open Access


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