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A protocol for the detection of genetic markers in saliva by polymerase chain reaction without a nucleic acid purifi cation step: Examples of SARS-CoV-2 and GAPDH markers

Kannan, S.; Ericsson, J.; Souchelnytskyi, N.; Souchelnytskyi, S. ( Danylo Halytskyi Lviv National Medical University , 2021 , Article)
Introduction. Polymerase chain reaction (PCR)-based diagnostic tests use purifi ed nucleic acids (NAs) from clinical samples. The NAs purifi cation step adds time, cost, and aff ects the quality of testing. The objective ...
Application of nucleic acid amplification tests in managing COVID-19 pandemic

Souchelnytskyi, S.; Souchelnytskyi, N. ( Danylo Halytskyi Lviv National Medical University , 2020 , Article)
Background. COVID-19 pandemic highlighted the importance of sensitive and specific tests that would be cost-efficient, fast and scalable. There are more than 200 COVID-19 detection tests available worldwide, with every ...
Cell-based diagnostic of bronchial carcinoma: Case study of a diagnostic value of tests of circulating tumor cells and organoid culture

Souchelnytskyi, N.; Kost, A.; Souchelnytskyi, S. ( Danylo Halytskyi Lviv National Medical University , 2020 , Article)
Diagnostic based on analysis of living tumor cells is frequently used in oncology. Circulating tumor cells in the blood and cells obtained from a tumor biopsy are used to access their carcinogenic properties and subsequently ...