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المؤلفBatista, Frederico M.
المؤلفChurcher, Allison M.
المؤلفManchado, Manuel
المؤلفLeitao, Alexandra
المؤلفPower, Deborah M.
تاريخ الإتاحة2018-09-19T08:55:50Z
تاريخ النشر2019
اسم المنشورAquaculture and Fisheries
المعرّفhttp://dx.doi.org/10.1016/j.aaf.2018.03.003
المعرّفhttp://dx.doi.org/10.1016/j.aaf.2018.03.003
الاقتباسBatista, F. M., Churcher, A. M., Manchado, M., Leitão, A., & Power, D. M. (2019). Uncovering the immunological repertoire of the carpet shell clam Ruditapes decussatus through a transcriptomic-based approach. Aquaculture and Fisheries, 4(1), 37-42.
معرّف المصادر الموحدhttp://hdl.handle.net/10576/11088
الملخصThe grooved carpet-shell clam Ruditapes decussatus is native to the Northern Atlantic and Mediterranean Sea and has a high commercial value. It is one of the main native bivalve species cultured in Europe. The main objective of the present study was to gain further insights into the immunological repertoire of R. decussatus through a transcriptomic approach. Pooled mantle samples of eight R. decussatus individuals were sequenced using Illumina platform. A total of 67 132 contigs with more than 800 bp were obtained. Manual annotation of these contigs revealed 146 immune-related genes. The gene families in which the highest number of immune-related genes was observed were: C1q domain-containing proteins (63), tumor necrosis factors (15) and toll-like receptors (TLRs, 10). A total of 5 359 putative single nucleotide polymorphisms (SNPs) were identified in the 146 immune-related genes. The density of SNPs ranged between 0.04 and 7.92 SNPs/100 bp. The highest and the lowest SNP density were observed in genes of the C1q domain-containing protein family. Due to the importance of TLRs in innate immunity, we focused our attention on these membrane receptors. Ten TLRs were identified based on protein domain organization. Phylogenetic analysis revealed that R. decussatus TLRs were diverse and only 3 showed orthology with TLRs of known immune functions in other bivalve species. Moreover, our analysis suggests that lineage restricted-expansions of TLRs occurred in all mollusc taxa analysed including in venerids.
راعي المشروعThis study was financially supported by the projects: AQUAGENET (SOE2/P1/E287) funded by the European Regional Development Fund within the program INTERREG IVB SUDOE; COMPETE/FEDER (FCOMP-01-0124-FEDER-010607); PTDC/MAR/103550/2008 and CCMAR/Multi/04326/2013 funded by the Foundation for Science and Technology (FCT). F.M.B. and A.M.C were supported by postdoctoral fellowship grants from FCT (SFRH/BPD/108591/2015 and SFRH/BPD/85408/2012). We would like to thank two anonymous referees for their constructive comments.
اللغةen
الناشرElsevier B.V. on behalf of KeAi Communications Co.
الموضوعRuditapes decussatus
Transcriptome
Immune genes
Toll-like receptors
العنوانUncovering the immunological repertoire of the carpet shell clam Ruditapes decussatus through a transcriptomic-based approach
النوعArticle
الصفحات37–42
رقم العدد1
رقم المجلد4
ESSN2468-550X
dc.accessType Open Access


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