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المؤلفAli M.H.M.
المؤلفRakib F.
المؤلفAbdelalim E.M.
المؤلفLimbeck A.
المؤلفMall R.
المؤلفUllah E.
المؤلفMesaeli N.
المؤلفMcNaughton D.
المؤلفAhmed T.
المؤلفAl-Saad K.
تاريخ الإتاحة2020-03-03T06:19:05Z
تاريخ النشر2018
اسم المنشورFrontiers in Neuroscience
المصدرScopus
الرقم المعياري الدولي للكتاب16624548
معرّف المصادر الموحدhttp://dx.doi.org/10.3389/fnins.2018.00647
معرّف المصادر الموحدhttp://hdl.handle.net/10576/13132
الملخصObjective: Stroke is the main cause of adult disability in the world, leaving more than half of the patients dependent on daily assistance. Understanding the post-stroke biochemical and molecular changes are critical for patient survival and stroke management. The aim of this work was to investigate the photo-thrombotic ischemic stroke in male rats with particular focus on biochemical and elemental changes in the primary stroke lesion in the somatosensory cortex and surrounding areas, including the corpus callosum. Materials and Methods: FT-IR imaging spectroscopy and LA-ICPMS techniques examined stroke brain samples, which were compared with standard immunohistochemistry studies. Results: The FTIR results revealed that in the lesioned gray matter the relative distribution of lipid, lipid acyl and protein contents decreased significantly. Also at this locus, there was a significant increase in aggregated protein as detected by high-levels A?1-42. Areas close to the stroke focus experienced decrease in the lipid and lipid acyl contents associated with an increase in lipid ester, olefin, and methyl bio-contents with a novel finding of A?1-42 in the PL-GM and L-WM. Elemental analyses realized major changes in the different brain structures that may underscore functionality. Conclusion: In conclusion, FTIR bio-spectroscopy is a non-destructive, rapid, and a refined technique to characterize oxidative stress markers associated with lipid degradation and protein denaturation not characterized by routine approaches. This technique may expedite research into stroke and offer new approaches for neurodegenerative disorders. The results suggest that a good therapeutic strategy should include a mechanism that provides protective effect from brain swelling (edema) and neurotoxicity by scavenging the lipid peroxidation end products.
راعي المشروعTexas Biomedical Research Institute, Technische Universitat Wien, Qatar University, Monash University, Qatar Foundation, National Institutes of Health, Qatar National Research Fund, Qatar Foundation
اللغةen
الناشرFrontiers Media S.A.
الموضوعBrain
FTIR imaging spectroscopy
Ischemic
LA-ICPMS
Lipid peroxidation
Neurodegeneration
Photothrombotic
Stroke model
العنوانFourier-transform infrared imaging spectroscopy and laser ablation -ICPMS new vistas for biochemical analyses of ischemic stroke in rat brain
النوعArticle
رقم العددSEP
رقم المجلد12
dc.accessType Abstract Only


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