Dual Role of Lanthanum Oxide Nanoparticles Functionalized Co-Polymeric Micelle for Extended Anti-Cancer Drug Delivery

Abstract

In this study, the paclitaxel (PTX) - loaded A?B type di-block polymeric micelles poly xylitol sebacate-poly methylmethacrylic acid/Lanthanum Oxide/paclitaxel (PXS-PMAA/LO/PTX) were designed and Lanthanum oxide nanoparticles were tagged on the surface of the micelle for the improvement of the antitumor drug efficiency in the cancer cells. LO nanoparticles influence the formation of sphere-shaped micelles and production of reactive oxygen species (ROS) to inhibite the cancer.Then, in-vitro drug release of PTX was carried out by UV-Vis spectroscopy and it was examined for pH depended drug releases. Cell uptake of the PTX-loaded micelles was investigated in human norml cell VERO and A549 cell line. The cytotoxicity study reveals that the PTX-loaded polymeric nano micelle has higher efficacy compared to free PTX. Living cell, cell apoptosis, ROS production and nuclear morphological changes were analyzed through Acridine Orange/Ethidium Bromide dual stain, cationic probe rhodamine 123, 2,7-dichlorofluorescene and Hoechst 33342 staining respectively. Hence, the PTX-loaded PXS-PMAA/LO carrier can act as potential drug carrier for cancer treatment.