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المؤلفKveton F.
المؤلفBlsakova A.
المؤلفLorencova L.
المؤلفJerigova M.
المؤلفVelic D.
المؤلفBlixt O.
المؤلفJansson B.
المؤلفKasak P.
المؤلفTkac J.
تاريخ الإتاحة2020-04-01T06:50:39Z
تاريخ النشر2019
اسم المنشورSensors (Switzerland)
المصدرScopus
الرقم المعياري الدولي للكتاب14248220
معرّف المصادر الموحدhttp://dx.doi.org/10.3390/s19245409
معرّف المصادر الموحدhttp://hdl.handle.net/10576/13580
الملخصThe study describes development of a glycan biosensor for detection of a tumor-associated antibody. The glycan biosensor is built on an electrochemically activated/oxidized graphene screen-printed electrode (GSPE). Oxygen functionalities were subsequently applied for covalent immobilization of human serum albumin (HSA) as a natural nanoscaffold for covalent immobilization of Thomsen-nouvelle (Tn) antigen (GalNAc-O-Ser/Thr) to be fully available for affinity interaction with its analyte—a tumor-associated antibody. The step by step building process of glycan biosensor development was comprehensively characterized using a battery of techniques (scanning electron microscopy, atomic force microscopy, contact angle measurements, secondary ion mass spectrometry, surface plasmon resonance, Raman and energy-dispersive X-ray spectroscopy). Results suggest that electrochemical oxidation of graphene SPE preferentially oxidizes only the surface of graphene flakes within the graphene SPE. Optimization studies revealed the following optimal parameters: activation potential of +1.5 V vs. Ag/AgCl/3 M KCl, activation time of 60 s and concentration of HSA of 0.1 g L−1. Finally, the glycan biosensor was built up able to selectively and sensitively detect its analyte down to low aM concentration. The binding preference of the glycan biosensor was in an agreement with independent surface plasmon resonance analysis.
راعي المشروعThe financial support received from the Slovak Scientific Grant Agency VEGA 2/0137/18 and 2/0090/16 from the Slovak Research and Development Agency APVV 17-0300 is acknowledged. This publication is the result of the project implementation: Centre for materials, layers and systems for applications and chemical processes under extreme conditions—Stage I, ITMS no.: 26240120007, supported by the ERDF. This publication was supported by Qatar University Collaborative Grant QUCG-CAM-19/20-2. The findings achieved herein are solely the responsibility of the authors.
اللغةen
الناشرMDPI AG
الموضوعBiosensor
Electrochemistry
Glycan
Graphene screen-printed electrodes
Tn antigen
العنوانA graphene-based glycan biosensor for electrochemical label-free detection of a tumor-associated antibody
النوعArticle
رقم العدد24
رقم المجلد19
dc.accessType Open Access


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