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AuthorAli M.H.M.
AuthorToor S.M.
AuthorRakib F.
AuthorMall R.
AuthorUllah E.
AuthorMroue K.
AuthorKolatkar P.R.
AuthorAl-Saad K.
AuthorElkord E.
Available date2020-04-23T14:21:35Z
Publication Date2019
Publication NameVaccines
ResourceScopus
ISSN2076393X
URIhttp://dx.doi.org/10.3390/vaccines7030109
URIhttp://hdl.handle.net/10576/14389
AbstractInteractions between programmed death-1 (PD-1) with its ligand PD-L1 on tumor cells can antagonize T cell responses. Inhibiting these interactions using immune checkpoint inhibitors has shown promise in cancer immunotherapy. MDA-MB-231 is a triple negative breast cancer cell line that expresses PD-L1. In this study, we investigated the biochemical changes in MDA-MB-231 cells following treatment with atezolizumab, a specific PD-L1 blocker. Our readouts were Fourier Transform Infrared (FTIR) spectroscopy and flow cytometric analyses. Chemometrical analysis, such as principal component analysis (PCA), was applied to delineate the spectral differences. We were able to identify the chemical alterations in both protein and lipid structure of the treated cells. We found that there was a shift from random coil and ?-helical structure to ?-sheet conformation of PD-L1 on tumor cells due to atezolizumab treatment, which could hinder binding with its receptors on immune cells, ensuring sustained T cell activation for potent immune responses. This work provides novel information about the effects of atezolizumab at molecular and cellular levels. FTIR bio-spectroscopy, in combination with chemometric analyses, may expedite research and offer new approaches for cancer immunology. - 2019 by the authors. Licensee MDPI, Basel, Switzerland.
SponsorDiabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110 Doha, Qatar Cancer Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110 Doha, Qatar Department of Chemistry and Earth Sciences, Qatar University (QU), P.O. Box 2713 Doha, Qatar Qatar Computing Research Institute, Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110 Doha, Qatar Qatar Environment & Energy Research Institute (QEERI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), P.O. Box 34110 Doha, Qatar Correspondence: mohamali@hbku.edu.qa (M.H.M.A.); eelkord@hbku.edu.qa (E.E.)
Languageen
PublisherMDPI AG
SubjectBiochemical alterations
Breast cancer
Chemometric analysis
FTIR
Spectroscopy
Tumor cells
TitleInvestigation of the effect of PD-L1 blockade on triple negative breast cancer cells using fourier transform infrared spectroscopy
TypeArticle
Issue Number3
Volume Number7
dc.accessType Open Access


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