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AuthorSaadaoui, Imen
AuthorAl Emadi, Maryam
AuthorBounnit, Touria
AuthorSchipper, Kira
AuthorAl Jabri, Hareb
Available date2021-07-05T11:03:42Z
Publication Date2016
Publication NameJournal of Applied Phycology
ResourceScopus
URIhttp://dx.doi.org/10.1007/s10811-015-0743-x
URIhttp://hdl.handle.net/10576/21185
AbstractThe Qatar University Culture Collection of Cyanobacteria and Microalgae (QUCCCM) is a unique resource containing a diverse collection of microalgae and cyanobacteria, isolated from the Qatar desert environment. In order to ensure maximum preservation of this resource, a number of cryopreservation techniques were applied to various strains, and the preservation effectiveness (cell viability and lipid productivity) was determined. The conditions tested were direct, passive, and freeze-cooling cryopreservation (technique), dimethyl sulfate and methanol (cryoprotectant), and 5 and 10% cryoprotectant concentrations over storage durations of up to 1 year. It was shown that the cryopreservation regime is strain dependent, and strains belonging to the same genera can have different requirements. On the other hand, neutral lipid estimation, via Nile red fluorescence determination of pre- and post-cryopreserved microalgae isolates, confirmed that the lipid production is affected by the applied cryopreservation method. Italian Society of Medical Radiology 2015.
Languageen
PublisherSpringer Netherlands
SubjectCryopreservation
Cryoprotectant
Long-termstorage
Microalgae
Neutral lipid
TitleCryopreservation of microalgae from desert environments of Qatar
TypeArticle
Pagination2233-2240
Issue Number4
Volume Number28
dc.accessType Abstract Only


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