Cardiac Myosin Binding Protein-C in Sprague Dawley (SD) Rats exposed to Sub-Chronic oral dose of Cadmium Chloride

Abstract

Cadmium is one of the major environmental pollutants associated with an increment in industrial and urbanization activities. It has health consequences when it reaches the human diet through the soil, water, and plant. Chronic exposure to cadmium has a serious effect on human health. It accumulates mainly in the liver and the kidney. In addition, it can accumulate in the different organs and tissues and exert its toxic effect on the body. Data generated from animal studies reported that elevated levels of cadmium in the blood lead to cardiovascular complications. Cardiac myosin-binding protein C (cMyBP-C) is associated with the myosin thick filament and is involved in cross-bridges cycling and regulation of muscle contraction. It is a reliable marker to indicate the status of cardiac contractility. Therefore, this study is carried out to investigate the effect of cadmium on levels of cMyBP-C during chronic cadmium toxicity. Male Sprague Dawley rats were subjected to a daily cadmium chloride dose (15ppm/Kg of body weight) for a period of 10 weeks. Then heart samples were collected and both gene and protein expression of cMyBP-C were analyzed. The data demonstrated that cadmium decreased cMyBP-C at the protein level and downregulated its expression at the gene level. Therefore, cMyBP-C can be used as a potential biomarker for cadmium cardiotoxicity