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المؤلفZupin, Luisa
المؤلفBarbi, Egidio
المؤلفSagredini, Raffaella
المؤلفOttaviani, Giulia
المؤلفCrovella, Sergio
المؤلفCelsi, Fulvio
تاريخ الإتاحة2022-01-23T06:54:30Z
تاريخ النشر2021-02-01
اسم المنشورJournal of Biophotonics
المعرّفhttp://dx.doi.org/10.1002/jbio.202000347
الاقتباسZupin, L, Barbi, E, Sagredini, R, Ottaviani, G, Crovella, S, Celsi, F. In vitro effects of photobiomodulation therapy on 50B11 sensory neurons: evaluation of cell metabolism, oxidative stress, mitochondrial membrane potential (MMP), and capsaicin-induced calcium flow. J. Biophotonics. 2021; 14:e202000347. https://doi.org/10.1002/jbio.202000347
الرقم المعياري الدولي للكتاب1864-063X
معرّف المصادر الموحدhttp://hdl.handle.net/10576/25696
الملخصThe analgesic properties of photobiomodulation therapy (PBMT) have been raising increasing interest in the clinical community due to the positive effects observed on patients, nevertheless the mechanistic basis of its action on peripheral sensory neurons remains still elusive. In this study, the effect of near-infrared (NIR) PBMT at 800 and 970 nm of wavelength was investigated on the 50B11 immortalized nociceptive sensory neuronal cell line by evaluating capsaicin-induced calcium flow and different markers correlated to mitochondria, that is, ATP, reactive oxygen species (ROS), and mitochondrial membrane potential (MMP). Calcium peak stimulated by capsaicin, the ligand of TRPV1 channel, was decreased in neurons pre-irradiated with the combination of the two wavelengths. Furthermore, delivering the 800 and 970 nm separately an increment of ATP, as well as MMP hyperpolarization were detected; notably, the 800 nm wavelength also increased ROS and O2− levels. Our findings, obtained on an in vitro model of nociception, show the positive effect of PBMT on two potential photo-targets of NIR light, namely the TRPV1 channel and the mitochondria.
راعي المشروعInstitute for Maternal and Child Health, IRCCS Burlo Garofolo/Italian Ministry of Health, Grant/Award Numbers: RC02/20, RC15/17
اللغةen
الناشرWiley
الموضوعcapsaicin-induced calcium flow
mitochondria
nociception
photobiomodulation
sensory neurons
TRPV1
العنوانIn vitro effects of photobiomodulation therapy on 50B11 sensory neurons: evaluation of cell metabolism, oxidative stress, mitochondrial membrane potential (MMP), and capsaicin-induced calcium flow
النوعArticle
رقم العدد2
رقم المجلد14
ESSN1864-0648
dc.accessType Full Text


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