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المؤلفPachetti, Maria
المؤلفD'Amico, Francesco
المؤلفZupin, Luisa
المؤلفLuppi, Stefania
المؤلفMartinelli, Monica
المؤلفCrovella, Sergio
المؤلفRicci, Giuseppe
المؤلفPascolo, Lorella
تاريخ الإتاحة2022-01-25T06:38:28Z
تاريخ النشر2021-12-04
اسم المنشورInternational Journal of Molecular Sciences
المعرّفhttp://dx.doi.org/10.3390/ijms222313134
الاقتباسPachetti, M.; D’Amico, F.; Zupin, L.; Luppi, S.; Martinelli, M.; Crovella, S.; Ricci, G.; Pascolo, L. Strategies and Perspectives for UV Resonance Raman Applicability in Clinical Analyses of Human Sperm RNA. Int. J. Mol. Sci. 2021, 22, 13134. https://doi.org/10.3390/ ijms222313134
معرّف المصادر الموحدhttp://hdl.handle.net/10576/25911
الملخصDeveloping a deeper knowledge about the impact of DNA and RNA epigenetic mutations on sperm production and fertilization performance is essential for selecting best quality samples in Assisted Reproductive Technologies (ART). Indeed, sperm RNAs adenine and guanine are likely to be methylated in low quality RNA sperm samples and their study requires the employment of techniques able to isolate high quality nucleic acids. UV resonance Raman spectroscopy represents a valuable tool that is able to monitor peculiar molecular modifications occurring predominantly in nucleic acids, being less sensitive to the presence of other biological compounds. In this work, we used an UV Resonance Raman (UVRR) setup coupled to a synchrotron radiation source tuned at 250 nm, in order to enhance sperm RNAs adenine and guanine vibrational signals, reducing also the impact of a fluorescence background typically occurring at lower energies. Despite that our protocol should be further optimized and further analyses are requested, our results support the concept that UVRR can be applied for setting inexpensive tools to be employed for semen quality assessment in ART.
اللغةen
الناشرMDPI
الموضوعRNA
UV Resonance Raman spectroscopy
sperm
العنوانStrategies and Perspectives for UV Resonance Raman Applicability in Clinical Analyses of Human Sperm RNA.
النوعArticle
رقم العدد23
رقم المجلد22
dc.accessType Open Access


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