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AuthorPachetti, Maria
AuthorD'Amico, Francesco
AuthorZupin, Luisa
AuthorLuppi, Stefania
AuthorMartinelli, Monica
AuthorCrovella, Sergio
AuthorRicci, Giuseppe
AuthorPascolo, Lorella
Available date2022-01-25T06:38:28Z
Publication Date2021-12-04
Publication NameInternational Journal of Molecular Sciences
Identifierhttp://dx.doi.org/10.3390/ijms222313134
CitationPachetti, M.; D’Amico, F.; Zupin, L.; Luppi, S.; Martinelli, M.; Crovella, S.; Ricci, G.; Pascolo, L. Strategies and Perspectives for UV Resonance Raman Applicability in Clinical Analyses of Human Sperm RNA. Int. J. Mol. Sci. 2021, 22, 13134. https://doi.org/10.3390/ ijms222313134
URIhttp://hdl.handle.net/10576/25911
AbstractDeveloping a deeper knowledge about the impact of DNA and RNA epigenetic mutations on sperm production and fertilization performance is essential for selecting best quality samples in Assisted Reproductive Technologies (ART). Indeed, sperm RNAs adenine and guanine are likely to be methylated in low quality RNA sperm samples and their study requires the employment of techniques able to isolate high quality nucleic acids. UV resonance Raman spectroscopy represents a valuable tool that is able to monitor peculiar molecular modifications occurring predominantly in nucleic acids, being less sensitive to the presence of other biological compounds. In this work, we used an UV Resonance Raman (UVRR) setup coupled to a synchrotron radiation source tuned at 250 nm, in order to enhance sperm RNAs adenine and guanine vibrational signals, reducing also the impact of a fluorescence background typically occurring at lower energies. Despite that our protocol should be further optimized and further analyses are requested, our results support the concept that UVRR can be applied for setting inexpensive tools to be employed for semen quality assessment in ART.
Languageen
PublisherMDPI
SubjectRNA
UV Resonance Raman spectroscopy
sperm
TitleStrategies and Perspectives for UV Resonance Raman Applicability in Clinical Analyses of Human Sperm RNA.
TypeArticle
Issue Number23
Volume Number22
dc.accessType Open Access


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