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AuthorVakilian S.
AuthorAlam K.
AuthorAl-Kindi J.
AuthorJamshidi-Adegani F.
AuthorRehman N.U.
AuthorTavakoli R.
AuthorAl-Riyami K.
AuthorHasan, Anwarul
AuthorZadjali F.
AuthorCsuk R.
AuthorAl-Harrasi A.
AuthorAl-Hashmi S.
Available date2022-05-21T10:18:26Z
Publication Date2021
Publication NameBiotechnology Journal
ResourceScopus
Identifierhttp://dx.doi.org/10.1002/biot.202100044
URIhttp://hdl.handle.net/10576/31259
AbstractBackground The development of anti-cancer drugs with the ability to inhibit brain metastasis through the blood-brain barrier (BBB) is substantially limited due to the lack of reliable in vitro models. Main Methods In this study, the Geltrex-based Transwell and microfluidic BBB models were applied to screen the effect of β-boswellic acid (β-BA) on the metastasis of MDA-MB-231 cells through the BBB in static and dynamic conditions, respectively. Major Results The toxicity assay revealed that β-BA deteriorates MDA-MB-231 cells, while β-BA had no detectable toxic effects on human umbilical vein endothelial cells (HUVECs) and astrocytes. Trans-endothelial electrical resistance evaluation showed sustainable barrier integrity upon treatment with β-BA. Vimentin expression in HUVECs, evaluated using western blot, confirmed superior barrier integrity in the presence of β-BA. The obtained results were confirmed using an invasion study with a cell tracker and a scanning electron microscope. β-BA significantly inhibited metastasis by 85%, while cisplatin (Cis), a positive control, inhibited cancer cell migration by 12% under static conditions. Upon applying a dynamic BBB model, it was revealed that β-BA-mediated metastasis inhibition was significantly higher than that mediated by Cis. Conclusions and Implications In summary, the current study proved the anti-metastatic potential of β-BA in both static and dynamic BBB models.
Languageen
PublisherJohn Wiley and Sons Inc
Subjectblood-brain barrier
boswellic acid
metastasis
microfluidics
Transwell
TitleAn engineered microfluidic blood-brain barrier model to evaluate the anti-metastatic activity of β-boswellic acid
TypeArticle
Issue Number10
Volume Number16
dc.accessType Abstract Only


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