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AuthorZehra M.
AuthorZubairi W.
AuthorHasan, Anwarul
AuthorButt H.
AuthorRamzan A.
AuthorAzam M.
AuthorMehmood A.
AuthorFalahati M.
AuthorAnwar Chaudhry A.
AuthorUr Rehman I.
AuthorYar M.
Available date2022-05-21T10:18:32Z
Publication Date2020
Publication NameInternational Journal of Nanomedicine
ResourceScopus
Identifierhttp://dx.doi.org/10.2147/IJN.S248911
URIhttp://hdl.handle.net/10576/31322
AbstractIntroduction: Diabetic wounds are challenging to treat due to a wide range of pathophysiological changes. Hypoxia is one of the predominant contributing factors of poor vascularization and chronicity in diabetic wounds. This study was designed to develop polycaprolactone (PCL)-based oxygen-releasing electrospun wound dressings and evaluate their efficacy for improved full thickness wound healing in diabetic rats. Methods: PCL-based oxygen releasing wound dressings were made using electrospinning technology. The developed dressings were characterized in terms of physical as well as biological properties both in vitro and in vivo. E-spun nanofibrous dressings were physically characterized with scanning electron microscopy, Fourier-transform infrared spectroscopy, and Energy-dispersive X-ray spectroscopy. To study the likely impact of the fabricated wound dressings in hypoxic conditions, HIF-1α expression analysis was carried out both at gene and protein levels. Wound dressings were further evaluated for their healing potential for extensive wounds in diabetic rat models. Results: The experimental results showed that the developed dressings were capable of continuously generating oxygen for up to 10 days. Cell studies further confirmed pronounced expression of HIF-1α at gene and protein levels in cells seeded on PCL-sodium percarbonate (SPC) and PCL scaffolds compared with the cells cultured on a tissue culture plate. Chorioallantoic membrane assay revealed the supportive role of oxygen releasing dressings on angiogenesis compared to the control group. Histological assessment of the regenerated skin tissues proved that full thickness wounds covered with SPC loaded PCL dressings had a comparatively better vascularized and compact extracellular matrix with completely covered thick epithelium. Discussion: The developed oxygen generating polymeric nanofibrous wound dressings could potentially be used as an envisioned approach for the efficient recovery of chronic diabetic wounds.
Languageen
PublisherDove Medical Press Ltd.
SubjectDiabetic wound healing
Electrospinning
Oxygenation
Polymeric nanofibers
Skin regeneration
TitleOxygen generating polymeric nano fibers that stimulate angiogenesis and show efficient wound healing in a diabetic wound model
TypeArticle
Volume Number15
dc.accessType Abstract Only


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