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المؤلفAl-Hamed F.S.
المؤلفAbu-Nada L.
المؤلفRodan R.
المؤلفSarrigiannidis S.
المؤلفRamirez-Garcialuna J.L.
المؤلفMoussa H.
المؤلفElkashty O.
المؤلفGao Q.
المؤلفBasiri T.
المؤلفBaca L.
المؤلفTorres J.
المؤلفRancan L.
المؤلفTran S.D.
المؤلفLordkipanidz� M.
المؤلفKaartinen M.
المؤلفBadran Z.
المؤلفTamimi F.
تاريخ الإتاحة2022-05-31T19:01:18Z
تاريخ النشر2021
اسم المنشورJournal of Clinical Periodontology
المصدرScopus
المعرّفhttp://dx.doi.org/10.1111/jcpe.13546
معرّف المصادر الموحدhttp://hdl.handle.net/10576/31808
الملخصAim: Platelet-rich plasma (PRP) is an autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP (L-PRP) composition on bone defect healing. Materials and Methods: L-PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP, double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro-CT and histology. Results: Double-spin L-PRP contained higher concentrations of platelets than single-spin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08 � 0.2 mm3) compared to single-spin L-PRP (1.42 � 0.27 mm3) or filtered L-PRP (1.38 � 0.28 mm3). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double-spin L-PRP than in those treated with single-spin or filtered L-PRP. Conclusion: The preparation method of L-PRP affects their composition and potential to regenerate bone
اللغةen
الناشرJohn Wiley and Sons Inc
الموضوعacid citrate dextrose
acid phosphatase tartrate resistant isoenzyme
alcohol
calcium
calcium chloride
carbon dioxide
carprofen
collagen
eg1160
eosin
hematoxylin
iron
magnesium
paraffin
potassium
selenium
serotonin
sodium
tolonium chloride
water
analgesia
animal experiment
animal model
animal tissue
Article
blood cell count
blood sampling
bone defect
bone mineral
bone regeneration
bone volume fraction
cell infiltration
centrifugation
citric acid cycle
cortical thickness (bone)
dehydration
dialysis
enzyme linked immunosorbent assay
erythrocyte
female
filtration
fracture healing
histology
histopathology
inductively coupled plasma mass spectrometry
leukocyte
liquid chromatography-mass spectrometry
lymphocyte
macrophage
mass fragmentography
mast cell
metabolite
micro-computed tomography
nonhuman
orthopedic surgery
osteoclast
oxidative stress
paraffin embedding
pentose phosphate cycle
platelet count
puncture
rat
serotonin blood level
thrombocyte rich plasma
tibia
trabecular number
trabecular separation
trabecular thickness
animal
connective tissue
Animals
Bone Regeneration
Collagen
Connective Tissue
Platelet-Rich Plasma
Rats
Tibia
العنوانDifferences in platelet-rich plasma composition influence bone healing
النوعArticle
الصفحات1613-1623
رقم العدد12
رقم المجلد48
dc.accessType Abstract Only


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